Development of a routine procedure for single nucleotide polymorphism marker design based on the T_m-shift genotyping method
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概要
- 論文の詳細を見る
To establish a routine procedure for SNP marker development, the previously reported T_m-shift SNP genotyping procedure was validated and extensively improved. The effect of introducing mismatched nucleotides into allele-specific primers, using alternative fluorescent dyes, and varying the DNA polymerase species on SNP discrimination was examined at more than 100 known SNP loci in a solanaceous crop, eggplant. As a result, it has been shown that a success rate over 80% can be achieved with T_m-shift SNP genotyping using directional trials with automatically designed primer sets and a routine protocol for the reaction condition tests.
- 日本育種学会の論文
著者
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Nunome T
National Inst. Vegetable And Tea Sci. Mie Jpn
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Nunome Tsukasa
Natl. Res. Inst. Veg. Ornam Plant & Tea.
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Nunome Tsukasa
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
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Nunome Tsukasa
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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Nunome Tsukasa
National Institute Of Vegetable And Tea Science (nivts)
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Yamaguchi Hirotaka
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
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Fukuoka Hiroyuki
Molecular Genetics and Physiology Research Team, National Institute of Vegetable and Tea Science (NI
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Miyatake Koji
Molecular Genetics and Physiology Research Team, National Institute of Vegetable and Tea Science (NI
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Negoro Satomi
Molecular Genetics and Physiology Research Team, National Institute of Vegetable and Tea Science (NI
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Ohyama Akio
Molecular Genetics and Physiology Research Team, National Institute of Vegetable and Tea Science (NI
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Ohyama A
National Res. Inst. Vegetables Ornamental Plants And Tea Mie Jpn
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Ohyama Akio
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
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Ohyama Akio
Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.
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Miyatake Koji
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
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Negoro Satomi
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
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Fukuoka H
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
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