Cloning of cDNA for a cell wall-bound acid invertase from tomato (Lycopersicon esculentum) and expression of soluble and cell wall-bound invertases in plants and wounded leaves of L. esculentum and L. peruvianum
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概要
- 論文の詳細を見る
A cDNA, Wiv-1, for an isozyme of acid invertase (EC 3.2.1.26) was cloned from wounded leaves of tomato (Lycopersicon esculentum). The encoded protein had a basic isoelectric point and strong similarity to the amino acid sequences of plant cell wall-bound invertases. The conserved sequence WECPD that is found in all plant cell wall-bound invertases was also found in the deduced protein. These results suggested that Wiv-1 encoded a cell wall-bound acid invertase of tomato. Wounding increased the levels of mRNAs for soluble and cell wall-bound invertases and the activities of these invertases in leaves of L. esculentum and of a related species, L. peruvianum. The induction of Aiv-1 mRNA for the soluble enzyme in wounded leaves was not very strong, while that of Wiv-1 mRNA for the wall-bound enzyme was prominent. The level of Aiv-1 mRNA reached a maximum 48 h after wounding while that of Wiv-1 mRNA continued to rise for up to 96 h. These findings suggested that the genes for the two isozymes responded independently to wounding. The levels in various organs of Aiv-1 and Wiv-1 mRNAs were higher in L. esculentum than in L. peruvianum. Possible roles of cell wall-bound acid invertase in wound response and in developing plant are discussed.
- 日本遺伝学会の論文
著者
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Hirai Masashi
National institute of Vegetable and Tea Science, National Agricultural Research Organization
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Hirai M
National Res. Inst. Vegetables Ornamental Plants And Tea Mie Jpn
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Hirai Masashi
Natl. Res. Inst. Veg. Ornam Plant & Tea.
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Nishimura Shigeo
National Research Institute Of Vegetables Ornamental Plants And Tea (nivot)
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OHYAMA Akio
National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
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Ohyama A
National Res. Inst. Vegetables Ornamental Plants And Tea Mie Jpn
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Ohyama Akio
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
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Ohyama Akio
Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.
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Nishimura S
National Research Institute Of Vegetables Ornamental Plants And Tea (nivot)
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Hirai Masashi
National Institute Of Vegetable And Tea Science (nivts):(present Address) Faculty Of Agriculture Kyo
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Ohyama Akio
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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