Conversion of RAPD Markers for a Clubroot Resistance Gene of Brassica rapa into Sequence-Tagged Sites (STSs)
スポンサーリンク
概要
- 論文の詳細を見る
We previously identiffed three random amplified polymorphic DNA (RAPD) markers linked to a major gene conferring clubroot resistance (CR) in Brassica rapa. The markers were cloned and sequenced. A pair of primers were designed for specific amplification of each marker. All three CR markers were specifically amplified as clear single and dominant bands. Identity of the loci of the amplified markers with the original RAPD markers was demonstrated using a segregating F_2 population. The marker bands can be amplified by at least two types of PCR machine. Therefore these three markers may be widely used as a reference to CR genes in the genome of B. rapa. Presence of the markers in turnips and Chinese cabbage cultivars was examined. Turnips showed a high degree of DNA polymorphism within cultivars. No relationship between CR and the presence of the marker bands was detected, while Chinese cabbage hybrid cultivars showed a rather low DNA polymorphism. One of the CR markers, RA12-75A was found to be useful for the breeding of CR Chinese cabbage, because no clubroot-susceptible cultivars of Chinese cabbage harbour this marker band. Use of these specifically amplified markers in marker-as-sisted selection (MAS) of Brassica crops is discussed.
- 日本育種学会の論文
著者
-
Hirai Masashi
National institute of Vegetable and Tea Science, National Agricultural Research Organization
-
Hirai M
National Research Institute Of Vegetables Ornamental Plants & Tea(nivot)
-
Hirai M
Kyoto Prefectural Univ. Kyoto Jpn
-
Hirai Masashi
Faculty Of Agriculture Kyoto Prefectural University
-
Kikuchi Motoyuki
National Institute of Fruit Tree Science, Ministry of Agriculture, Forestry and Fisheries
-
Kuginuki Yasuhisa
National Institute of Vegetable and Tea Science
-
Ajisaka Hidetoshi
National Research Institute of Vegetables, Ornamental Plants & Tea (NIVOT)
-
Kikuchi Motoyuki
National Institute Of Fruit Tree Science Ministry Of Agriculture Forestry And Fisheries:(present Add
-
Kikuchi Motoyuki
National Research Institute Of Vegetables Ornamental Plants & Tea(nivot):fukushima Prefecture Ag
-
Kuginuki Y
National Research Institute Of Vegetables Ornamental Plants & Tea(nivot)
-
Hirai Masashi
National Institute Of Vegetable And Tea Science (nivts):(present Address) Faculty Of Agriculture Kyo
-
Ajisaka Hidetoshi
National Research Institute Of Vegetables Ornamental Plants & Tea(nivot)
関連論文
- Inheritance of Few-pungent Trait in Chili Pepper 'S3212' (Capsicum frutescens)
- Development of Microsatellite Markers in Melon ( Cucumis melo L. ) and Their Application to Major Cucurbit Crops
- Fertile somatic hybrids between Solanum integrifolium and S. sanitwongsei (syn. S. kurzii) as candidates for bacterial wilt-resistant rootstock of eggplant
- Isolation and Characterization of a 60 kDa 2, 4-D-Binding Protein from the Shoot Apices of Peach Trees(Prunus persica L.) : It Is a Homologue of Protein Disulfide Isomerase
- ISOLATION OF NEW AUXIN-BINDING PROTEINS FROM SHOOT APEX OF PEACH
- Purification and Properties of an Auxin-Binding Protein from the Shoot Apex of Peach Tree
- Characteristics of Microsatellites in Brassica rapa Genome and their Potential Utilization for Comparative Genomics in Cruciferae
- Modulation of Gene Expression in Isolated Nuclei by Auxin-Binding Proteins
- Enhanced Resistance against a Fungal Pathogen Sphaerotheca humuli in Transgenic Strawberry Expressing a Rice Chitinase Gene
- Development of Breeding Materials of Transgenic Tomato Plants with a Truncated Replicase Gene of Cucumber Mosaic Virus for Resistance to the Virus
- Mapping of Fruit Shape and Color Development Traits in Eggplant(Solanum melongena L.)Based on RAPD and AFLP Markers
- Cloning of cDNA for a cell wall-bound acid invertase from tomato (Lycopersicon esculentum) and expression of soluble and cell wall-bound invertases in plants and wounded leaves of L. esculentum and L. peruvianum
- Suppression of Acid Invertase Activity by Antisense RNA Modifies the Sugar Composition of Tomato Fruit : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM
- A novel cDNA clone for acid invertase in tomato fruit
- Mapping of QTLs for Bolting Time in Brassica rapa (syn. campestris) under Different Environmental Conditions
- Highly Regenerative Cultivars in Microspore Culture in Brassica Oleracea L. var. capitata
- Mapping of Loci Affecting the Cultural Efficiency of Microspore Culture of Brassica rapa L. syn. campestris L. using DNA Polymorphism
- Development of a Genetic Marker Linked to the Tendril Trait of Sweet Pea ( Lathyrus odoratus L. )
- Conversion of RAPD Markers for a Clubroot Resistance Gene of Brassica rapa into Sequence-Tagged Sites (STSs)
- Utilization of DNA Polymorphism in Vegetable Breeding(Utilization of DNA-Marker for Breeding and Classification in Horticultural Plants, For Further Development of Horticulture in East Asia)
- Resistance Responses in Chinese Cabbage to Clubroot Disease (Plasmodiophora brassicae)
- Genetic Analysis of Clubroot Resistance in Brassica Crops
- Introducing an Antisense Gene for a Cell-Wall-Bound Acid Invertase to Tomato (Lycopersicon esculentum) Plants Reduces Carbohydrate Content in Leaves and Fertility
- Association between Water Status and Sucrose Metabolism in Cell Suspension Culture of Carrot