Development of Microsatellite Markers in Bunching Onion (Allium fistulosum L.)
スポンサーリンク
概要
- 論文の詳細を見る
Since few genetic markers are available in bunching onion, many DNA markers are needed for the construction of a primary basis for the breeding of this crop. We report here the development of microsatellite markers in bunching onion. A size-fractionated genomic library was constructed from genomic DNA of bunching onion cv. 'Kujo Futo' and screened with a mixture of (GA)_<15> and (GT)_<15> oligonucleotide probes. From approximately 180,000 clones we isolated and sequenced 94 positive clones. A total of 52 clones were identified as having microsatellite repeats. Of them 49 had a GT motif, while only one had a GA motif, which is known to be more frequent than GT in most plant species. This is the first report of the abundance of GT over GA in Allium spp. Of 50 specific PCR primer pairs designed for the microsatellite-containing clones, 33 primer pairs amplified polymorphic loci in nine cultivars of bunching onion, 115 alleles detected in total. These results indicate that microsatellites with dinucleotide motifs, especially with GT, are promising sources of highly informative genetic markers in bunching onion.
- 日本育種学会の論文
著者
-
Suwabe Keita
National Institute of Vegetable and Tea Science
-
Suwabe K
National Inst. Vegetable And Tea Sci. Mie Jpn
-
Suwabe Keita
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
-
Song Yeon-sang
National Institute Of Vegetable And Tea Science (nivts) National Agriculture And Food Research Organ
-
Nunome Tsukasa
National institute of Vegetable and Tea Science, National Agricultural Research Organization
-
Nunome T
National Inst. Vegetable And Tea Sci. Mie Jpn
-
Nunome Tsukasa
Natl. Res. Inst. Veg. Ornam Plant & Tea.
-
Nunome Tsukasa
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
-
Nunome Tsukasa
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
-
Nunome Tsukasa
National Institute Of Vegetable And Tea Science (nivts)
-
Ohara Takayoshi
National Institute of Vegetable and Tea Science
-
WAKO Tadayuki
National Institute of Vegetable and Tea Science (NIVTS)
-
Wako Tadayuki
National Institute Of Vegetable And Tea Science (nivts) National Agriculture And Food Research Organ
-
Kojima Akio
National Institute of Vegetable and Tea Science
-
Kojima Akio
National Institute Of Vegetable And Tea Science (nivts) National Agriculture And Food Research Organ
-
Nunome Tsukasa
National Institute Of Vegetable And Tea Science
-
Kojima Akio
Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
-
Wako Tadayuki
Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
関連論文
- Mapping of QTLs controlling root shape and red pigmentation in radish, Raphanus sativus L.
- Construction of a Molecular Linkage Map of Radish (Raphanus sativus L.), Based on AFLP and Brassica-SSR Markers
- Development of Microsatellite Markers in Melon ( Cucumis melo L. ) and Their Application to Major Cucurbit Crops
- Influences of Rootstock Cultivar and Storage on the Texture of Cucumber Fruits
- Inheritance of Resistance to Cucumber Green Mottle Mosaic Virus in Cucumis melo L. 'Chang Bougi'
- Molecular and biochemical identification of alien chromosome additions in shallot (Allium cepa L.. Aggregatum group) carrying extra chromosome(s) of bunching onion (A. fistulosum L.)
- Detection of Textural Difference between Cultivars of Bunching Onion using the Device for Acoustic Measurement of Food Texture
- Development of a routine procedure for single nucleotide polymorphism marker design based on the T_m-shift genotyping method
- Characterization of the promoter of the Wiv-1 (Lin6) gene encoding a wound-inducible cell wall-bound acid invertase in tomato
- Development of Microsatellite Markers in Bunching Onion (Allium fistulosum L.)
- Characteristics of Microsatellites in Brassica rapa Genome and their Potential Utilization for Comparative Genomics in Cruciferae
- Characterization of Trinucleotide Microsatellites in Eggplant
- Analysis of transgenic tobacco harbouring cDNA for L-galactonolactone dehydrogenase feom sweet potato
- L-Galactono-γ-lactone Dehydrogenase from Sweet Potato : Purification and cDNA Sequence Analysis
- cDNA CLONING OF L-GALACTONO-LACTONE DEHYDROGENASE FROM SWEET POTATO
- The Mechanism of Polyembryonic Seed Set in Paspalum notatum
- The Mechanisms of Appearance of Aposporous Initial Cell and Apomictic Embryo Sac Formation in Paspalum notatum
- Considerable Heterogeneity in Commercial F_1 Varieties of Bunching Onion (Allium fistulosum) and Proposal of Breeding Scheme for Conferring Variety Traceability Using SSR Markers
- Development of Breeding Materials of Transgenic Tomato Plants with a Truncated Replicase Gene of Cucumber Mosaic Virus for Resistance to the Virus
- Mapping of Fruit Shape and Color Development Traits in Eggplant(Solanum melongena L.)Based on RAPD and AFLP Markers
- Development of microsatellite markers in cultivated and wild species of sections Cepa and Phyllodolon in Allium
- Development of a simple and rapid extraction method of glucosinolates from radish roots
- Strawberry cultivar identification based on hypervariable SSR markers
- Microstructure of a Brassica rapa genome segment homoeologous to the resistance gene cluster on Arabidopsis chromosome 4
- Modes of inheritance of two apomixis components, diplospory and parthenogenesis, in Chinese chive (Allium ramosum) revealed by analysis of the segregating population generated by back-crossing between amphimictic and apomictic diploids
- Classification and identification of bunching onion (Allium fistulosum) varieties based on SSR markers
- Activation of Immune Responses in Mice by an Oral Administration of Bunching Onion (Allium fistulosum) Mucus
- Characterization of Fruit-Type Sucrose Synthase Gene Promoters Isolated from Tomato and Japanese Pear