Considerable Heterogeneity in Commercial F_1 Varieties of Bunching Onion (Allium fistulosum) and Proposal of Breeding Scheme for Conferring Variety Traceability Using SSR Markers
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概要
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DNA markers are powerful tools for verifying the varietal identity and genetic homogeneity of F_1 hybrid seeds. F_1 varieties are becoming increasingly prevalent in bunching onion (Allium fistulosum L.) production in Japan because of the high uniformity of agronomic traits. However, bunching onion is an allogamous crop and suffers from severe inbreeding depression when selfed. It is considered that not only open-pollinated varieties but also the parental lines of F_1 hybrids should maintain a certain degree of average heterozygosity and hence genetic heterogeneity. In the present study, the genetic homogeneity of eight bunching onion varieties, including six F_1 hybrids, was evaluated using 14 SSR markers. Two or more polymorphic alleles were detected at all of the SSR loci examined in each variety. The number of alleles detected in the eight varieties ranged from 3 to 7 among the 14 SSR loci, and the polymorphism information content from 0.41 to 0.76. All the varieties examined displayed very low degrees of uniformity at all of these polymorphic loci. Based on these results, it may be impossible to determine an appropriate genotypic identity for any of the existing bunching onion varieties. To facilitate and enhance the accuracy of variety identification, we proposed here an "SSR-tagged breeding" scheme in which the plants homozygous at a few SSR loci would be selected out of a foundation seed field. This scheme may enable to achieve efficient variety identification and purity determination of F_1 seeds not only in bunching onion but also in any allogamous crops exhibiting severe inbreeding depression.
- 日本育種学会の論文
著者
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TSUKAZAKI Hikaru
National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
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Song Yeon-sang
National Institute Of Vegetable And Tea Science (nivts) National Agriculture And Food Research Organ
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WAKO Tadayuki
National Institute of Vegetable and Tea Science (NIVTS)
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Wako Tadayuki
National Institute Of Vegetable And Tea Science (nivts) National Agriculture And Food Research Organ
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FUKUOKA Hiroyuki
National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
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Kojima Akio
National Institute of Vegetable and Tea Science
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Kojima Akio
National Institute Of Vegetable And Tea Science (nivts) National Agriculture And Food Research Organ
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Yamashita Ken-ichiro
National Institute of Vegetable and Tea Science (NIVTS), National Agriculture and Food Research Orga
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Fukuoka Hiroyuki
National Inst. Of Vegetable And Tea Sci.
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Tsukazaki Hikaru
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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Yamashita Ken-ichiro
Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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Tsukazaki Hikaru
Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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Kojima Akio
Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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Wako Tadayuki
Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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