Development of a Genetic Marker Linked to the Tendril Trait of Sweet Pea ( Lathyrus odoratus L. )

概要

論文の詳細を見る
A sweet pea ( Lathyrus odoratus L. ) cultivar, 'Grace', which has tendrils, was crossed with 'Snoopea purple' lacking tendrils. The resultant F_2 population was used to identify RAPD ( random amplified polymorphic DNA ) markers linked to a tendril gene. The presence of tendrils was found to segregate in a dominant fashion. A total of 302 random primers were used to screen a pair of bulked DNA samples of theF_2 plants. Only two primers, WB32 and WB67, showed polymorphism between the bulked samples. The former generated a DNA fragment specific to the bulked sample with tendrils, while the latter amplified a fragment in the bulked sample without tendrils. Segregation of these RAPD markers was examined in the F_2 population. One of them, WB32a was found to be linked to the tendril gene. The marker was then cloned and sequenced. A pair of primers was designed for specific amplification of this marker. The primer pairs amplified a clear and dominant band, SWB32a, and the band was specific to individuals with tendrils. The linkage between the marker, SWB32a and the gene for tendrils was demonstrated in the F_2 population in a distance of 7.7 cM. Use of this genetic marker in the breeding of sweet pea cultivars without tendrils was discussed.