2 Prevotella nigrescens 由来赤血球凝集因子の性状について (第446回大阪歯科学会例会)

概要

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We isolated a hemagglutinin from Prevotella nigrescens strain E4 by mechanical shearing, ultrasonication and gel filtration. Hemagglutinating activity was eltuted in the second peak on a Sepharose CL-4 B column. The activity was sensitive to trypsin, chymotrypsin, protease, β-glucosidase and various detergents, but resistant to heat at 80℃ for 10 min. Addition of _L-lysine and _L-arginine caused hemagglutination inhibition, although addition of _L-fucose, _L-rhamnose and maltose increased the activity. The active fraction reacted with antiserum against hemagglutinin from Prevotella intermedia E 18. These results indicate that this hemagglutinin is a protein-polysaccharide complex and is distributed on most of the cell surface of Prevotella intermedia and Prevotella nigrescens.
大阪歯科学会の論文
1996-03-25