1 In situ PCR法のhuman papilloma virus ゲノムへの適用 (第441回大阪歯科学会例会)

概要

論文の詳細を見る
Human papilloma virus (HPV) infection has been implicated in the etiology of oral cancer. The frequency of detection of HPV in oral cancer tissues ranges from 0 to 100%. Some of this variability can be attributed to the various methods of detection. Therefore, we developed a highly sensitive HPV detection system using the in situ polymerase chain reaction (PCR). It makes use of the extreme sensitivity of PCR and the localizing ability of in situ hybridization. The HPV primers (MY 09 and MY 11) used in this study target the highly conserved L1 gene coding for the major component of viral capsid protein, and amplify 450 base-pair product from more than 50 HPV types. A number of control experiments were performed to prevent false positive reactions and to find the optimal conditions. In situ PCR appears to be an effective technique for examining HPV in oral lesions.
大阪歯科学会の論文
1995-06-25