Ethanol-Soluble and Insoluble Fractions of Humic Substances in Soil Fulvic Acids
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概要
- 論文の詳細を見る
Humic substances in fulvic acids (FAs) obtained as polyvinylpyrrolidone (PVP)-adsorbed fractions were further fractionated based on their solubility in ethanol. Elementary composition, IR spectra, UV-Vis spectra, saccharide contents, molecular size distribution, and ^13C-NMR spectra were clearly different between the ethanol-soluble and the insoluble fraction. The yield of the ethanol-soluble fractions exceeded 80%. These fractions showed higher aromatic- and carbonyl-C contents than the insoluble fractions. The differences in the signal intensities of phenol or aryl ether C_1 and methoxyl groups in the ^13C-NMR spectra (lowland paddy soil > brown forest soil > ando soil) as well as the aromatic-C contents (lowland paddy soil > ando soil > brown forest soil) of these fractions among the three soils suggested that the content and composition of the aromatic components varied depending on the soil types. Saccharides and nitrogen compounds were mainly distributed in the ethanol-insoluble fractions and it was suggested that these fractions were composed of humic substances combined with polysaccharides. The weight average molecular weights of these fractions which were 25,000-30,000, were about two times as large as those of the ethanol-soluble fractions. The lower log K values of the insoluble fractions than those of the soluble fractions indicated the presence of structural differences in the aromatic components between the two fractions.
- 社団法人日本土壌肥料学会の論文
著者
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Watanabe Akira
Laboratory of Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya Univer
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Kuwatsuka Shozo
Laboratory Of Soil Science Faculty Of Agriculture Nagoya University
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Watanabe A
Research Division For Development Of Anti-infection Agents Institute Of Development Aging And Cancer
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Watanabe Akira
Laboratory Of Radiation Biology Faculty Of Science University Of Tokyo:(present) Research Institute
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Watanabe Akira
Laboratory Of Soil Science School Of Agriculture Nagoya University
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Watanabe Akira
Laboratory Of Bioindustrial Genomics Graduate School Of Agricultural Science Tohoku University
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