RNA synthesis in germinating red bean seeds

概要

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Nucleic acids from ^<32>P-labelled germinating red bean seeds were investigated by means of MAK column chromatography. 1) In cotyledons, synthesis of D-RNA occur-red in the early stages of germination, 3 to 24 hr after the onset of imbibition. ^<32>P was also incorporated into rRNA continuously at rather a moderate rate. DNA-RNA hybridization experiments revealed that the proportion of heterogeneous RNA (D-RNA) to rRNA decreased gradually. Nucleotide analysis suggested that tRNA was synthesized de novo, and that its CCA-end exchange was remarkable at early stages of germination. 2) In embryos, however, the incorporation of ^<32>P into rRNA was very much greater than into D-RNA, and the exchange reaction at CCA-end of tRNA was not detected. The role of D-RNA, found in cotyledons in the initial stages of germination, was discussed.
日本植物生理学会の論文