Ethanol-Soluble and Insoluble Fractions of Non-Humic Substances in Soil Fulvic Acids
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概要
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The non-humic substances in fulvic acids (FAs) of three Japanese soils were obtained as the non-absorbed fraction through a column of insoluble polyvinyl-pyrrolidone (PVP), and this fraction was further fractionated based on the solubility in ethanol. Although the yield of the ethanol-soluble fractions from the PVP-non-adsorbed FAs was 5-7%, the analytical data indicated that their chemical structure was clearly different from that of the ethanol-insoluble fractions. The ethanol-soluble fractions were characterized by low average molecular weights, low saccharide contents and strong peaks for aliphatic C-H, alcoholic C-O, and carboxyl groups in the IR spectra. The ^13C-NMR spectrum of this fraction in the ando soil that was determined quantitatively also showed strong peaks for the long alkyl chain, secondary alcohol or aliphatic ether, and carboxyl groups. The main constituents of the ethanol-insoluble fractions consisted of polysaccharides. Contents and composition of saccharides, however, varied depending on the soil types. Pentose content of the insoluble fraction was very high in the lowland paddy soil, and the uronic acid content was higher in the ando soil than in the other two soils. The carbohydrate-C content of the insoluble fractions estimated from ^^13C-NMR spectra was in the order of lowland paddy soil, ando soil, and brown forest soil. The aliphatic-C content was in the opposite order. These findings suggested that abundant plant components remained in the FA of the lowland paddy soil while many aliphatic components were incorporated into the FA of the brown forest soil.
- 社団法人日本土壌肥料学会の論文
著者
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Watanabe Akira
Laboratory of Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya Univer
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Kuwatsuka Shozo
Laboratory Of Soil Science Faculty Of Agriculture Nagoya University
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Watanabe Akira
Laboratory Of Radiation Biology Faculty Of Science University Of Tokyo:(present) Research Institute
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Watanabe Akira
Laboratory Of Bioindustrial Genomics Graduate School Of Agricultural Science Tohoku University
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Watanabe Akira
Laboratory Of Bioindustrial Cenomics Graduate School Of Agricultural Science Tohoku University
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