ヒト,ウサギおよびウシ臓器由来の組織因子を用いた第VII因子アミダーゼ活性の比較

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A comparison was made among factor VII amidolytic activities on a chromogenic substrate assay using tissue factors from human brain (HB-TF), rabbit brain/lung (RB/L-TF) and bovine brain (BBITF). Factor VII amidolytic activities using HB-TF showeｄ linearity between absorbance (A₄₀₅nm) and diluent concentration of normal pooled-plasma on log-log paper, but not RB/L-TF and BB-TF. In normal individuals, factor VII amidolytic activities using three tissue factors were　given close agreement (p>0.05). In patients receiving stabilized oral anticoagulation, factor VII amidolytic activities using RB/L-TF (r=0.828) and BB-TF (r=0.742) correlated well with that using HB-TF, respectively. Factor VII amidolytic activities using RB/L-TF (62.7±23.8%.) Were somewhat higher than those from HB-TF (56.1±22.50/.) and BB-TF (56.1±22.7%). In all kinds of disease containing liver disease, factor VII amidolytic activities using RB/LTF (r=0.771) and BB-TF (r=0.769) correlated well with that using HB-TF, although factor VII amidolytic activities using BB-TF (108.8±34.7%) were somewhat higher than those from HB-TF (92.8±35.2%) and RB/L-TF (95.0±31.7%), In eleven factor VII deficiencies with CRM⁻, factor VII amidolytic activities using RB/L-TF (3.9±3.0%) was somewhat higher than those using remaining two tissue factors, HB-TF (2.1±1.8%) and BB-TF (2.4±1.4%).
信州大学の論文