Bromchlorphenol Blueを用いた血液標本の迅速鉄染色法の検討

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A representative evaluation of the iron status of patients with refractory anemia, characterized by ineffective erythropoiesis and abnormal iron metabolism, is possible by detecting bone marrow and blood smears stained for iron. For iron-staining on bone marrow and blood smears, Prussian blue reaction has been conventional. We clinically evaluated the rapid method which Kass et al. had developed the cytochemical iron-staining using bromchorphenol blue (dibrom-dichlorphenol sulfonphthalein). This new method may be useful for iron-staining on bone marrow and blood smear. It is much more simple and rapid requiring only about one minute. The positive ratio and scoring for this iron-staining on bone marrow and blood smears from patients with various blood diseases correlated well with those for the traditional method by prussian blue reaction. It seemed to be kept strictly pH. 3.2 in the stain solution for the best effect.
信州大学の論文