抗サイログロブリン抗体および抗マイクロゾーム抗体測定法の研究

概要

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SERODIA AMC (PA-AMC) and SERODIA ATG (PA-ATG) with gelatin particle agglutination (PA) are most widely used for the determination of anti-thyroglobulin antibody (TgAb) and anti-microsomal antibody (McAb) in laboratory medicine. However, the positive incidence of Hasimoto's disease in these measurements was 80% with PA-AMC and 30% with PA-ATG respectively. We tried to define the causes of the difference produced between PA-ATG and PA-AMC by follwing experiments. 1. An Inhibition test in which Tg antigen was added to both PA-AMC and PA-ATG reagent system in order to identify positive incidence. 2. An enzyme immunoassay (EIA) in which purified Mc antigen (absolutely free from Tg) and purified Tg antigen were used in order to identify non-specific positive reactions. Twenty-six percent of the false positive sera appeared in PA-AMC positive sera. However, no false positive sera were observed in PA-ATG positive sera. These results suggested that Mc antigen in reagent could have been contaminated with Tg Antigen and also McAb could have a cross reactivity to Tg. We concluded that Tg added PA-AMC system, Inhibition method, or Thyroid peroxidase (TPO) method were most suitable for the determination of serum McAb.
信州大学の論文