甲状腺穿刺液サイログロブリンのレクチン親和測定法 : 甲状腺分化癌診断の可能性

概要

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The sugar chain residue of thyroglobulin produced in thyroid cancer (C-Tg) has a modified structure compared with that in the normal thyroid (N-Tg). We have measured the remaining unbound-thyroglobulin (ub-Tg) in thyroid specimens by an enzyme immunoassay (EIA) after treatment with concanavalin A (ConA) lectin, taking advantage of different affinity of C-Tg to lectin. Three thyroid tissue specimens ( 1 normal, 1 papillary carcinoma, 1 benign goitor) and 28 thyroid aspirated specimens (16 papillary carcinoma and 12 benign goiter) were analyzed. Tg to lectin ratio of 1 : 3 was at the zenith of the ub-Tg％. Thus, ConA at a ratio of 1 : 3 were used for subsequent studies. The ub-Tg value for papillary carcinoma tissue was significantly higher than the benign goiter. Five of 16 specimens obtained by aspiration from thyroid cancer manifested elevated ub-Tg levels after ConA treatment. Thus, measurement of ub-Tg in thyroid aspirated specimens after treatment with ConA may prove to be useful for the diagnosis of thyroid cancer.
信州大学の論文