堆肥から分離した Clostridium thermosaccharolyticum F-9 のβ-グルコシーダゼの性質

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Nine strains of cellulolytic-thermophilic anaerobes were isolated from hot springs and composts andanalyzed for their productivity of β-glucosidase (β-D-Glucoside glucohydrolase EC 3. 2. 1. 21). Among them, strain F-9 showed the highest β-glucosidase activity, and this strain was identified as a member of Clostridium thermosaccarolyticum on taxonomical characteristics. The intracellular β-glucosidase of the bacterium was purified 40-fold with a recovery of 6%. The purified enzyme preparation was chromatographically homogeneous. The molecular weight ofabout 350,000 was estimated by gel filtration using Sephadex G-150, Bio-Gel p-300 and Sepharose 4B.Its isoelectric point was estimated to be 4.6. The activity of the enzyme for cellobiose was very muchlower than for p-nitrophenyl β-D-glucoside. The enzyme did not hydrolyse maltose, lactose, carboxy-methyl-cellulose and cellulose. It was strongly inhibited by D-glucono-δ-lactone and was sensitive tothiol reagents.
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