ホウレンソウ葉肉プロトプラストの初期培養条件の改良

概要

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In order to improve the plating efficiency on spinach mesophyll protoplast culture, various conditions were examined with four cvs. of spinach, four cvs. of chard and one cv. of tablebeet. 1. Mesophyll protoplasts of these test plants were isolated from the in vitro-grown young seedlings by treatment of the enzyme solution containing 2 % Cellulase Onozuka RS, 0.5 % Cellulase Onozuka R-10,0.5 % Macerozyme R-10,0.02 % Pectolyase Y-23,CPW salts, 0.1 % MES and 0.5 M sorbitol (pH 5.8) for 4-11 hours. 2. The cell division and colony formation of spinach cv. 'SHINNIPPON' occurred only in a modified MS medium (No. 3) containing 1 mg/l NAA, 1 mg/l 2,4-D and 1 mg/l BA in five combinations of growth regulators. 3. The cell division and colony formation of protoplasts only occurred in all spinach cvs., but not in chard and tablebeet in No. 3 medium. 4. The frequency of cell division and colony formation in spinach cv. 'F_1 SHINRYOKU' increased to DMSO concentration. Plating efficiency of the medium containing 1.0 % DMSO (14.7 %) was much higher than that of the medium without DMSO (3.2 %) . 5. We now plan to induce plant regeneration from the thus obtained callus.
恵泉女学園園芸短期大学の論文