<原著>ヒト腫瘍細胞株と大腸癌組織におけるlamininの免疫組織学的研究および転移との関連

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To clarify the relation between basement membrane components and the invasion and metastases of cancer, clinical materials from 176 patients with colorectal cancer and 13 tumor cell lines cultured in collagen gel were used in an immunohistochemical analysis, based upon the ABC method, of laminin (LN), a component of the basement membrane. A retrospective study revealed that LN activity is primarily found on the basal side of cancer cell nests which form glandular structures. No LN activity was found in 25.4% of the primary lesions without liver metastases, but was seen frequently in 76.7% of those cases which did involve liver metastases. LN activity was found not only on the basal side of the glandular structures but was also seen around small cancer nests which were invading the stroma without any such glandular structure. In those areas of vascular invasion, the LN positive layers in both the cancer nests and venous basal sides were neither destroyed nor had disappeared from the primary lesions. Apparently when the layers had adhered and become fused, it was obvious that the cancer cell nests had intensively invaded into the venous vessels and the LN positive layers had become detached from each other. In the liver metastatic lesions, LN activity was found evenly throughout the basal part of the glandular structures in 94.1% of the cases. In the prospective study for predicting whether liver metastases were formed by means of LN activity in the primary lesions of colorectal cancer or not, sensitivity was 88.2% and specificity was 78.8%, which indicate that LN staining is useful as ascreening test for the recurrence of liver metastases. In the limited study involving human tumor cell lines cultured in collagen gel, COLO 201,WiDr, C-1 and MKN-28・6TG cell lines formed basement-like structures with LN being localized on the margin facing the gel. This indicates that cancer cells secrete LN.
近畿大学の論文
1992-06-25

<原著>ヒト腫瘍細胞株と大腸癌組織におけるlamininの免疫組織学的研究および転移との関連

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