<原著>癌を中心とした大腸組織における酵素抗体法によるCEA(carcino-embryonic antigen)の細胞組織化学的研究

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The immunofluorescent technique which has been widely used as a method of histochemical demonstration for carcino-embryonic antigen (CEA) is not capable of detailed morphological studies. By using the immunoperoxidase technique, therefore, we have made the cytochemical and histochemical studies on CEA of normal mucosa, hyperplastic mucosa, adenoma and cancer of the large intestine. (1) With regard to tissue fixation methods, frozen sections showed the strongest CEA activity followed by freeze-substitution method. Considerably satisfactory findings were obtained as regards these fixation methods. On the contrary, cold-acetone fixation was accompanied by the decrease in CEA activity as well as remarkable distortion of tissue structure, and therefore were not suitable for morphological studies of CEA. Formalin-fixation was also accompanied by the decrease in CEA activity, but this fact demonstrated that the use of formalin-fixation permitted distinction between cancer and normal mucosa on the basis of the pattern of CEA staining. (2) The localization of CEA on frozen sections of normal mucosa, adenoma and cancer of the large intestine was observed in the glycocalyx covering the cell membrane bordering the lumina of the glands and in the mucigen granules of the goblet cell. These two types of CEA were qualitatively different. It is assumed that the former is the CEA with high cancerspecificity and that the latter is the substance referred to as CEA-F which exists in feces. (3) Histochemical studies of CEA on formalin fixed sections were conducted. The 232 specimens included normal mucosa, hyperplastic mucosa, adenoma and cancer of the large intestine. Among 77 large bowel carcinomas, all of 67 differentiated adenocarcinomas showed positive staining for CEA, which were located at the apical border of the glandular cells. In 3 signet ring cell carcinomas, CEA was observed as an intracellular granules. While, one case of squamous cell carcinoma proved for CEA. None of the normal mucosae were positive for CEA. The mucosae adjacent to carcinoma or adenoma were not commonly positive for CEA, and in positive cases CEA staining was demonstrated in the upper half of the tubules. In 156 polyps of the large intestine, there existed a definite correlation between the intensity of CEA staining and the epithelial atypism, and the incidence of positivity tended to become higher as atypism of adenoma became more severe. The immunoperoxidase staining for CEA in the mucigen granules of the goblet cells was only positive as frozen sections and not after formalin-paraffin processed. (4) This technique has higher sensitivity as compared with immunofluorescent technique, and is capable of detailed morphological studies. It is verified that this technique will serve as a useful supplementary technique to produce CEA which is specifically present in cancer tissues, for the purpose of purified CEA.
近畿大学の論文
1981-03-25

<原著>癌を中心とした大腸組織における酵素抗体法によるCEA(carcino-embryonic antigen)の細胞組織化学的研究

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