<原著>酵素抗体法によるCEAの細胞組織化学的研究(I)

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The immunofluorescent technic applied as a method of histochemical demonstration of carcino-embryonic antigen (CEA) is not capable of detailed morphological studies. Therefore, we conducted the cytochemical and histochemical study on CEA using immunoperoxidase technic. (1) With regard to fixation methods, frozen sections showed the strongest CEA activity followed by freeze-substitution method. Considerably satisfactory findings were obtained as regards these fixation methods. On the contrary, formalin fixation and cold-acetone fixation were accompanied by the decrease in CEA activity as well as remarkable distortion of tissue structure, and therefore were not suitable for morphological study of CEA. (2) The localization of CEA in the tissues of normal mucosa, adenoma and cancer of the large intestine was observed in the glycocalyx covering the cell membrane bordering the lumina of the glands and in the mucigen granules of the goblet cell. These two types of CEA were qualitatively different. It was assumed that the former is the CEA with high cancer-specificity and that the latter is substance referred to as CEA which exists in feces. This technic has higher sensitivity as compared with immunofluorescent technic and is capable of detailed morphological studies. It was verified that this technic will serve as a useful supplementary technic to produce CEA which is specifically present in cancer tissues, for the purpose of purifying CEA.
近畿大学の論文
1978-12-25

<原著>酵素抗体法によるCEAの細胞組織化学的研究(I)

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