<原著>骨格筋切断後における筋線維再生現象の組織学的観察

概要

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It has been already reported that myofibers have the ability to regenerate after necrosis. If the basement membranes of necrotic myofibers remain after an injury of muscle tissue, they are used as scaffolding for myofiber regeneration. However the details of mechanism of muscle regeneration, including muscle spindles, are not throughly clarified yet. In the present study, myotubes budding from both stumps and their basement membranes were histologically studied after muscle transection. Extensor digitorum longus muscles of Wistar rats were first transected, and then resected 2,4,7,14,or 120 days after the transection. Sections of the muscle, 5μm in thickness, were cut with a cryostat, and each was observed light microscopically after HE, reticulin staining, routine ATPase, and acetylcholine esterase reaction. As a result, growing points of myotubes were found to be surrounded by the newly formed basement membranes. Therefore it was considered that regenerating myofibers had presynthesized the new basement membrane for use as scaffolding. Regenerating muscle spindles were discovered surrounded by those regenerating myofibers. Although the capsules of the spindles had been believed not to show remarkable changes, the capsules showed conspicuous thickening in this experiment.
近畿大学の論文
1988-03-25