クロレラエキスに含まれる血圧降下物質の分離・同定(A 基礎的研究)(II 予防および治療)

概要

論文の詳細を見る
We attempted isolation of the hypotensive substance contained in hot water extracts of chlorella raw cells. When chlorella extracts were fractionated by gel filtration on Sephadex G-15 (column 4.5×60 cm), fraction E (elution volume 1,200 to 1,560 ml) showed a depressive effect. The blood pressure of SHR and SHRSP showed falls of about 60 mmHg at 1 hour after intravenous administration and of 30 to 40 mmHg at 2 hours after intragastric administration. Fraction E was subsequently purified by passage through a column of Sephadex G-10,and then crystallized from ethanol. Both the crystalline compound and the mother liquor showed hypotensive effects, but the effect of the latter was more potent. The crystalline compound was finally identified as adenosine by comparing it with an authentic sample of adenosine, on the basis of UV, IR, NMR and MS spectral data. The mother liquor also contained adenosine, but the result of an amino acid analysis indicated the presence of a considerable amount of glycine. Therefore, the mother liquor was considered to be a mixture of adenosine and the unknown compound containing glycine. As a result, the hypotensive effect of fraction E on SHR and SHRSP may be ascribable to adenosine or to an oligopeptide.
近畿大学の論文
1980-09-25