Establishment of new method for analysis of starch contents and varietal differences in soybean seeds
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概要
- 論文の詳細を見る
Aside from a report claiming that soybean seeds contain less than 1% starch, little is actually known about the genetic variation of the starch content in this important crop species. We used a starch-iodine test to identify varieties with high starch content in a soybean germplasm collection and found a total of 34 cultivars that showed a strongly positive reaction (dark color). For a more accurate quantitation of starch contents, we established a new method using a heat-resistant α-amylase and dinitrosalicylic acid (DNS) reagent. We compared the DNS method and the standard method using glucose-oxidase (GOD) with a known amount of starch as a standard. We showed that the DNS method generated results that were very highly correlated with the GOD method. In addition, we found the new method to be easier and faster to implement than the GOD method. Using the DNS method, we found several accessions in our soybean germplasm whose starch contents were 2-7%.
- 2010-06-01
著者
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Abe Jun
Graduate School Of Agriculture Hokkaido Univ.
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原田 久也
National Inst. Of Agrobiological Sciences
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Harada K
Graduate School Of Science And Technology Chiba University
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Harada Kyuya
National Institute Of Agrobiological Resources Ministry Of Agriculture Forestry And Fisheries
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Harada Kyuya
Graduate School Of Science And Technology Chiba University
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Kitamura Keisuke
Graduate School Of Agriculture Hokkaido Univ.
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Kitamura Keisuke
Research Faculty Of Agriculture Hokkaido University
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YAMADA Tetsuya
Research Faculty of Agriculture, Hokkaido University
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Yamada Tetsuya
Laboratory Of Plant Genetics And Evolution Graduate School Of Agriculture Hokkaido University
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Yamada Tetsuya
National Institute Of Crop Science (nics)
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Yamada Tetsuya
Graduate School Of Agriculture Hokkaido University
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Abe Jun
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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JEONG Woo-Hyeun
Graduate School of Agriculture, Hokkaido University
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Jeong Woo-hyeun
Graduate School Of Agriculture Hokkaido University
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HARADA Kyuya
National Institute of Agrobiological Resources
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