Genetic Analysis of High α-tocopherol Content in Soybean Seeds
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概要
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Tocopherols, known as members of vitamin E, are the major lipophilic antioxidants in soybean. Since soybean seeds contain a low concentration of α-tocopherol (α-Toc), the tocopherol isoform with the highest vitamin E activity, some breeding efforts have been implemented to increase the α-tocopherol content in the seeds. In the present study, we used F_2 seed and F_2 plant (F_3 seeds) populations derived from a cross between a variety with a high α-tocopherol content, Keszthelyi A.S. (α-Toc 20-30%) and a Japanese cultivar, Ichihime (α-Toc<10%) for genetic analysis of the high α-tocopherol concentration. There was no significant correlation between the α-tocopherol and total tocopherol contents in the F_2 plant (F_3 seeds) population, suggesting that the α-tocopherol and total tocopherol contents were regulated independently in the seeds. Therefore, it may be possible to increase the α-tocopherol concentration without affecting the total tocopherol content. Broad-sense heritability value for the α-tocopherol concentration trait was estimated to be 0.645 in the F_2 seeds, suggesting that the α-tocopherol concentration trait was highly heritable. Chi-square goodness-of-fit test for evaluating the relationship between genotype of certain SSR markers and the α-tocopherol concentration showed that the Sat_243 and Sat_167 markers (linkage group K) were significantly associated with the α-tocopherol concentration (P<0.05). These markers could be used for marker-assisted selection to breed soybean cultivars with high α-tocopherol concentrations. Analysis of the F_2 seeds and the F_2 plants (F_3 seeds) for tocopherol composition showed that there was a significantly negative correlation between the α-tocopherol and γ-tocopherol concentrations, suggesting that the activity of γ-tocopherol methyltransferase, the enzyme that converts γ-tocopherol to α-tocopherol, is high in individuals with a high α-tocopherol concentration.
- 日本育種学会の論文
著者
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Ujiie Aya
Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University
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Yamada Tetsuya
Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University
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Kitamura Keisuke
Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University
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Kitamura Keisuke
Laboratory Of Plant Genetics And Evolution Graduate School Of Agriculture Hokkaido University
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Thuy Le
Laboratory Of Plant Genetics And Evolution Graduate School Of Agriculture Hokkaido University
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Ujiie Aya
Laboratory Of Plant Genetics And Evolution Graduate School Of Agriculture Hokkaido University
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Kitamura Keisuke
Graduate School Of Agriculture Hokkaido Univ.
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Kitamura Keisuke
Research Faculty Of Agriculture Hokkaido University
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YAMADA Tetsuya
Research Faculty of Agriculture, Hokkaido University
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Yamada Tetsuya
Laboratory Of Plant Genetics And Evolution Graduate School Of Agriculture Hokkaido University
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Yamada Tetsuya
National Institute Of Crop Science (nics)
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Yamada Tetsuya
Graduate School Of Agriculture Hokkaido University
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Dwiyanti Maria
Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University
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Dwiyanti Maria
Laboratory Of Plant Genetics And Evolution Graduate School Of Agriculture Hokkaido University
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YAMADA Tetsuya
Laboratory of Food and Environmental Hygiene, Department of Veterinary Medicine, Gifu University
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