Comparison of Methods for Isolating Exosomes from Bovine Milk
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概要
- 論文の詳細を見る
Four methods were evaluated for isolating exosomes from bovine milk: (1) ExoQuick precipitation, (2) ultracentrifugation with ExoQuick precipitation, (3) ultracentrifugation with density gradient centrifugation, and (4) human milk exosome isolation. Methods 1 and 4 failed due to differences between bovine and human milk. Exosomes were efficiently isolated by ultracentrifugation with either ExoQuick precipitation (method 2) or density gradient centrifugation (method 3). The highest yield of exosomes was achieved using ultracentrifugation with ExoQuick precipitation, whereas higher quality exosome isolation with intact morphological structures was achieved by ultracentrifugation with density gradient centrifugation.
- 日本獣医学会の論文
著者
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Ishiguro Naotaka
Laboratory Of Food And Environmental Hygiene Faculty Of Applied Biological Sciences Gifu University
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Matsuda Tsukasa
Graduate School Of Applied Molecular Bioagricultural Sciences Nagoya University
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Inoshima Yasuo
Laboratory Of Food And Environmental Hygiene Veterinary Medicine Faculty Of Applied And Biological Sciences Gifu University
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MATSUDA Tsukasa
Graduate School of Bio-agricultural Sciences, Nagoya University
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YAMADA Tetsuya
Laboratory of Food and Environmental Hygiene, Department of Veterinary Medicine, Gifu University
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