Production of transgenic plants and their early seed set in Japanese soybean variety, Kariyutaka
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概要
- 論文の詳細を見る
A soybean variety, Kariyutaka was found to form much more precocious flower buds than those of the other six genotypes used in this study when in vitro cultured at 26°C with 16/8 h light/dark regime (fluorescent tubes at an intensity of about 100 μmols s−1 m−2). In addition, it was a suitable genotype for soybean transformation because it was highly sensitive to Agrobacterium and it exhibited excellent shoot regeneration. Its transformation frequency (about 1%) was comparable to that of a variety Thorne, which is known as one of the suitable cultivars for Agrobacterium-mediated transformation. Because transgenic shoots of Kariyutaka formed in vitro precocious flower buds, T1 seeds were obtained in only six months after co-cultivation with Agrobacterium. A few copy numbers of transgenes were inheritable to T1 generations. T1 plants also produced seed within 2–3 months when grown at the same regime as in vitro culture. Hence Agrobacterium-mediated transformation using Kariyutaka can be recommended as a model method for the rapid development of transgenic soybean lines.
- 日本植物細胞分子生物学会の論文
- 2007-12-01
著者
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KITAMURA Keisuke
Graduate School of Agriculture, Hokkaido University
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Kitamura Keisuke
Graduate School Of Agriculture Hokkaido University
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Ishimoto Masao
National Agricultural Res. Center For Hokkaido Region
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Kitamura Keisuke
Graduate School Of Agriculture Hokkaido Univ.
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Yamada Tetsuya
Graduate School Of Agriculture Hokkaido University
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Sato Hiroshi
Graduate School of Humanities and Sciences, Ochanomizu University
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KITA Yohichi
Graduate School of Agriculture, Hokkaido University
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Kita Yohichi
Graduate School Of Agriculture Hokkaido University
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Sato Hiroshi
Graduate School Of Economics Hitotsubashi University
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Sato Hiroshi
Graduate School of Agriculture, Hokkaido University
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