Identification of AFLP Markers Tightly Linked to the Gene for Deficiency of the 7S Globulin in Soybean Seed and Characterization of Abnormal Phenotypes Involved in the Mutation

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Soybean seed protein consists of two major groups, 7S globulin (β-conglycinin) and 11S globulin (glycinin). A mutant line, cgdef mutant, lacking three major 7S globulin subunits, α, α' and β, was developed by γ-ray irradiation. The deficiency was controlled by a single recessive allele, cgdef. This mutation is associated with cgdef syndrome, morphological abnormality, sterility and lethality. The objective of this study is to perform detailed genetic analysis of the Cgdef locus using AFLP technique. We identified two AFLP markers that cosegregated with the Cgdef using 167 segregants obtained by selfing of a plant heterozygous for cgdef. After cloning and sequencing the two AFLP framents, they were converted to sequence-characterized amplified regions (SCARs). Linkage analysis of the two AFLP-SCAR markers in 103 F_2 plants derived from a cross between Mo-shi-dou Gong 503 and the plant heterozygous for cgdef showed that these markers are tightly linked to Cgdef at a distance of 1.1±0.12 cM. Furthermore, in the F_2 population, all phenotypes of the cgdef syndrome including leaf abnormality, sterility and lethality were recombined with cgdef. This indicates that the cgdef syndrome is derived not from pleiotropy of the cgdef locus but from close linkage with some other unidentified gene(s). The markers might be useful for positional cloning of Cgdef.
日本育種学会の論文