A Judgment on Postmortem Aging in Longissimus Dorsi Based on a Peptide Substrate Library
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概要
- 論文の詳細を見る
We attempted to develop a method to determine easily and effectively the degree of postmortem aging of pork longissimus dorsi (LD) by measuring the activity of proteases in the LD using fluorogenic peptide substrates. LD was used to measure the change with time in the protease activity detected with these substrates. Determining the variations within the LD muscles, strong positive correlations were found between changes in hardness and fluorescence intensities against Ac-Ala-MCA, Ac-Met-MCA, Ac-Ser-MCA, Ac-Thr-MCA, and Ac-Ala-Phe-MCA (P<0.005), and strong negative correlations were found between changes in total amounts of free amino acids and Ac-Ala-MCA, Ac-Met-MCA, Ac-Ser-MCA, Ac-Thr-MCA, and Ac-Ala-Phe-MCA (P<0.001). Negative correlations were also observed between changes in the amounts of free Ala, Arg, Lys, Leu, Met, Phe, and Tyr and the fluorescence intensities against Ala, Arg, Lys, Leu, Met, Phe, and Tyr-MCA respectively (P<0.001).
- 社団法人 日本農芸化学会の論文
- 2006-12-23
著者
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MORIMATSU Fumiki
Research and Development Center, Nippon Meat Packers, Inc.
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NISHINO Norikazu
Department of Applied Chemistry, Faculty of Engineering, Kyushu Institute of Technology
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MURAKAMI Hiroshi
Research Institute for Food Science, Kyoto University
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Nishino Norikazu
Department Of Biological Functions And Engineering Graduate School Of Life Science And Systems Engin
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Nishino Norikazu
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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ONITSUKA Eiichiro
Research and Development Center, Nippon Meat Packers, Inc.
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OKUMURA Tomoyuki
Research and Development Center, Nippon Meat Packers, Inc.
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Morimatsu Fumiki
Research And Development Center Nippon Meat Packers Inc.
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Okumura Tomoyuki
Research And Development Center Nippon Meat Packers Inc.
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Onitsuka Eiichiro
Research And Development Center Nippon Meat Packers Inc.
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Murakami Hiroshi
Research And Development Center Nippon Meat Packers Inc.
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Onitsuka Eiichiro
Res. And Dev. Center Nippon Meat Packers Inc.
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