A Novel Method to Estimate the Contribution of the Vapor Activity of Essential Oils in Agar Diffusion Assay

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概要

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• By the combined use of agar diffusion, agar vapor and agar vapor-inhibitory assays, contribution of the vapor activity of essential oils was quantitatively estimated. The test organisms were Trichophyton mentagrophytes and Aspergillus fumigatus. Agar vapor assay was used to confirm the vapor activity of the oils. The parameter delta defined as a contribution index of the vapor activity was calculated by (1- b-c/a-c) × 100, where a is inhibitory diameter in the diffusion assay, b is inhibitory diameter in the vapor-inhibitory assay and c is diameter of the sealed ring in the vapor-inhibitory assay (21 mm). Many of the essential oils examined showed a delta value near 100, thus providing the major contribution of the vapor activity to the inhibitory diameter. Essential oils containing aldehyde as major constituent showed low delta value, indicating the major inhibition was due to agar diffusion. Major essential oil components behaved similarly; the delta value was increased in the following order: aldehyde<phenol<alcohol<ester, oxide, hydrocarbon, indicating the enhanced contribution of the vapor activity in that order.

• 日本医真菌学会の論文
• 2006-04-30

著者

• Abe Shuichi

  山形県立新庄病院

• INOUYE Shigeharu

  Teikyo University Institute of Medical Mycology

• YAMAGUCHI Hideyo

  Teikyo University Institute of Medical Mycology

• Abe S

  Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University

• Abe Shigeru

  Departments Of Radiology And Cardiology Yamagata Prefectural Shinjo Hospital

• Abe S

  Teikyo Univ. Tokyo Jpn

• UCHIDA Katsuhisa

  Teikyo University Institute of Medical Mycology

• Yamaguchi Hideyo

  Teikyo Univ. Inst. Of Medical Mycology Tokyo Jpn

• Yamaguchi Hideyo

  Departments Of Microbiology And Immunology University Of Teikyo School Of Medicine

• UCHIDA Katsuhisa

  Institute of Medical Mycology, Graduate School of Medical Science, Teikyo University

• MARUYAMA Naho

  Teikyo University Institute of Medical Mycology

• ABE Shigeru

  Teikyo University Institute of Medical Mycology

• Mimaki Yoshihiro

  Lab. Of Medicinal Plant Sci. School Of Pharmacy Tokyo Univ. Of Pharmacy And Life Sci. Jpn

• Abe S

  Japan Poliomyelitis Res. Inst. Tokyo Jpn

• Uchida K

  Teikyo University Institute Of Medical Mycology

• Haraguchi Yuji

  Department Of Hygiene And Virology Gunma University School Of Medicine

• Abe Shigeru

  Teikyo University Institute Of Medical Mycology And Genome Research Center Teikyo University

• Inouye Shigeharu

  Teikyo Univ. Inst. Of Medical Mycology

• Inouye S

  Pharmaceutical Research Center Meiji Seika Kaisha Ltd.

• Uchida K

  Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University

• Uchida Katsuhisa

  Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University

• Abe Sigeru

  Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University

• Yamaguchi Hideyo

  Teikyo University Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University

• Abe Shigeru

  Teikyo University Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University

• Yamaguchi Hideyo

  Teikyo Univ. Inst. Medical Mycology

• Uchida Katsuhisa

  Teikyo Association of Medical Mycology

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