A One-Enzyme PCR-RFLP Assay for Identification of Six Medically Important Candida Species

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概要

• 論文の詳細を見る

• Early identification of Candida isolates to the species level is necessary for effective antifungal therapy, and can also facilitate control of hospital infections. Phenotype-based methods for identifying Candida species are often difficult and time-consuming. Molecular biological techniques provide a useful alternative approach. In the present study, the ITS1-5.8S-ITS2 regions of fungal rRNA genes were amplified with universal primers in 20 standard strains. Digestion of the PCR products with one restriction enzyme, MspI, allowed discrimination of medically important Candida species, including C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, and C. guilliermondii. Using this method, we successfully identified 137 clinical isolates of Candida. Among them, C. albicans was identified as the most common species, followed by C. parapsilosis, C. tropicalis, C. glabrata, C. krusei, and C. guilliermondii. This method is a simple, rapid, and cost-effective method for differentiation between species that is applicable in clinical laboratories.

• 日本医真菌学会の論文
• 2006-07-31

著者

• YAMAGUCHI Hideyo

  Teikyo University Institute of Medical Mycology

• YAMAGUCHI HIDEYO

  Institute of Medical Mycology, Teikyo University

• Yamaguchi Hideyo

  Teikyo Univ. Inst. Of Medical Mycology Tokyo Jpn

• Yamaguchi Hideyo

  Departments Of Microbiology And Immunology University Of Teikyo School Of Medicine

• MAKIMURA Koichi

  Institute of Medical Mycology, Graduate School of Medical Science, Teikyo University

• Makimura Koichi

  Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University

• Makimura Koichi

  Teikyo University Institute Of Medical Mycology And Genome Research Center Teikyo University

• Makimura K

  Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University

• Yamaguchi H

  Department Of Internal Medicine National Cancer Center Hospital

• Haraguchi Yuji

  Department Of Hygiene And Virology Gunma University School Of Medicine

• MIRHENDI Hossein

  Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Hea

• KHORAMIZADEH Mohamadreza

  Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences

• Mirhendi Hossein

  Department Of Medical Parasitology And Mycology School Of Public Health And Institute Of Public Heal

• Khoramizadeh Mohamadreza

  Department Of Pathobiology School Of Public Health Tehran University Of Medical Sciences

• Yamaguchi Hideyo

  Teikyo University Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University

• Yamaguchi Hideyo

  Institute Of Medical Mycology Teikyo University

• Yamaguchi Hideyo

  Institute Of Medical Mycology And Genome Research Center Teikyo University

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