Impact of free-living amoebae on presence of Parachlamydia acanthamoebae in the hospital environment and its survival in vitro without requirement for amoebae.
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概要
- 論文の詳細を見る
Parachlamydia acanthamoebae is an obligately intracellular bacterium that infects free-living amoebae and is a potential human pathogen in hospital-acquired pneumonia. We examined whether the presence of P. acanthamoebae is related to the presence of Acanthamoeba in an actual hospital environment and assessed the in vitro survival of P. acanthamoebae. Ninety smear samples were collected between November 2007 and March 2008 (trial 1, n 52) and between October 2008 and February 2009 (trial 2, n 38) from the floor (dry conditions, n 56) and sink outlets (moist conditions, n 34) of a hospital. The prevalences of P. acanthamoebae DNA in the first and second trials were 64.3% and 76%, respectively. The prevalences of Acanthamoeba DNA in the first and second trials were 48% and 63.1%, respectively. A statistical correlation between the prevalence of P. acanthamoebae and that of Acanthamoeba was found (trial 1, P 0.011; trial 2, P 0.022), and that correlation increased when samples from just the dry area (floor smear samples, P 0.002) were analyzed but decreased when samples from a moist area were analyzed (P 0.273). The in vitro experiment showed that, without Acanthamoeba, P. acanthamoebae could not survive in dry conditions for 3 days at 30°C or 15 days at 15°C. Thus, both organisms were coincidentally found in an actual hospital environment, with the presence of Acanthamoeba having a significant effect on the long-term survival of P. acanthamoebae, suggesting that this potential human pathogen could spread through a hospital environment via Acanthamoeba.
- American Society for Microbiologyの論文
著者
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Yamaguchi H
Teikyo Univ. Inst. Medical Mycology
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Yamaguchi H
Department Of Internal Medicine National Cancer Center Hospital
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Yamaguchi H
Department Of Emergency And Critical Care Medicine Nippon Medical School
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MATSUO Junji
Department of Medical Laboratory Sciences, Faculty of Health Sciences, Hokkaido University
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FUKUMOTO Tatsuya
Hokkaido University Hospital
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YOSHIDA Mitsutaka
Division of Ultrastructural Research, Juntendo University Graduate School of Medicine
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Yamaguchi Hiroyuki
Department Of Medical Laboratory Sciences Faculty Of Health Sciences Hokkaido University
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Matsuo Junji
Department Of Medical Laboratory Sciences Faculty Of Health Sciences Hokkaido University
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SAKAI HARUNA
Division of Biomedical Imaging Research, Juntendo University Graduate School of Medicine
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NAKAMURA SHINJI
Division of Biomedical Imaging Research, Juntendo University Graduate School of Medicine
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