Combined Effect of Heat, Essential Oils and Salt on the Fungicidal Activity against Trichophyton mentagrophytes in Foot Bath
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概要
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This work was originally undertaken to determine the effective conditions of essential oils against Trichophyton mentagrophytes in vitro for the treatment of tinea pedis in a foot bath. Agar blocks implanted with T. mentagrophytes were immersed in 0.1% aqueous agar containing two-fold dilutions of essential oils with or without sodium chloride at 27°C, 37°C and 42°C for 10 and 20 min. The number of surviving mycelia on the agar blocks was determined from the standard curves of the colony diameter and original inocula of the conidia. At the same time, the thermal effect on the cellular morphology was examined using SEM. Most fungal mycelia (99.7%) were killed after treatment at 42°C for 20 min without essential oil. The fungicidal activity of essential oils was markedly enhanced by treating at 42°C for 20 min as compared with that at 27°C, showing 1/4∼ 1/32-fold reduction of minimum fungicidal concentration (MFC to kill 99.99%) . The order of the fungicidal activity of 11 essential oils was oregano, thyme thymol, cinnamon bark > lemongrass > clove, palmarose, peppermint, lavender > geranium Bourbon, tea tree > thyme geraniol oils. MFCs were further reduced to 1/2∼1/8 by the addition of 10% sodium chloride. The salt effect was explained, at least partly, by an increase in mycelial adsorption of antifungal constituents in the presence of sodium chloride. Considerable hyphal damage was done at 27°C by the essential oils, but no further alteration in morphology of the hyphae treated at 42°C with or without oil was observed by SEM. The inhibitory effect of heat and oils was also observed against mycelia of T. rubrum and conidia of T. mentagrophytes. Thermotherapy combined with essential oils and salt would be promising to treat tinea pedis in a foot bath.
著者
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YAMAGUCHI Hideyo
Teikyo University Institute of Medical Mycology
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UCHIDA Katsuhisa
Teikyo University Institute of Medical Mycology
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Yamaguchi Hideyo
Teikyo Univ. Inst. Of Medical Mycology Tokyo Jpn
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ABE Shigeru
Teikyo University Institute of Medical Mycology
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Nishiyama Yukihiro
Laboratory Of Virology Research Institute For Disease Machanism And Control
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HASUMI Yayoi
Teikyo University Institute of Medical Mycology
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Inouye Shigeharu
Teikyo Univ. Inst. Of Medical Mycology
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Uchida K
Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University
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Uchida Katsuhisa
Institute Of Medical Mycology Graduate School Of Medical Science Teikyo University
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Nishiyama Yayoi
Teikyo Univ. Inst. Medical Mycology Tokyo
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Yamaguchi Hideyo
Teikyo Univ. Inst. Medical Mycology
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Uchida Katsuhisa
Teikyo Association of Medical Mycology
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