Lipid peroxidation of a human hepatoma cell line (HepG2) after incorporation of linoleic acid, arachidonic acid, and docosahexaenoic acid
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概要
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Lipid peroxidation of human heptoma cell line, HepG2, after incorporation of linoleic acid (LA), arachidonic acid (AA), and docosahexaenoic acid (DHA) was measured with a fluorescent probe and gas chromatography–mass spectrometry (GC–MS) analysis. The analysis with a fluorescent probe showed that incorporation of each polyunsaturated fatty acid (PUFA) enhanced the cellular lipid peroxidation level, but there was little difference in the effect of LA, AA, or DHA on the enhancement of cellular lipid peroxidation. The fluorescent analysis also showed that the addition of H2O2 (0.5 mM) enhanced the cellular lipid peroxidation levels in LA and AA supplemented cells as compared with those without H2O2. However, the enhancement of lipid peroxidation by H2O2 was not observed in DHA-supplemented cells. The same result was obtained in the GC–MS analysis of total amounts of monohydroperoxides (MHP) formed in the cellular phospholipid oxidation. In this case, the main source for MHP was LA in LA-, AA-, and DHA-supplemented cells. A significant amount of AA–MHP and a small amount of DHA–MHP were observed in AA- and DHA-supplemented cells respectively. GC–MS analysis also indicated the specific positional distribution of DHA–MHP isomers. The isomers were formed only by hydrogen abstraction at the C-18 (16-MHP + 20-MHP; 46.5%), C-6 (4-MHP + 8-MHP; 38.5%), and C-12 (10-MHP + 14-MHP; 15.1%) positions, but not at the C-9 or C-15 positions.
- 2005-03-23
著者
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HOSOKAWA Masashi
Laboratory of Biofunctional Material Chemistry, Division of Marine Bioscience, Graduate School of Fi
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MIYASHITA Kazuo
Laboratory of Biofunctional Material Chemistry, Division of Marine Bioscience, Graduate School of Fi
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Kobayashi Hidetaka
Laboratory Of Biofunctional Material Chemistry Division Of Marine Bioscience Graduate School Of Fish
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Hosokawa Masashi
Laboratory Of Biofunctional Material Chemistry Division Of Marine Bioscience Graduate School Of Fish
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Hosokawa Masashi
Laboratory Of Biofunctional Material Chemistry Division Of Marine Bioscience Craduate School Of Fish
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ARASEKI Mina
Laboratory of Biofunctional Material Chemistry, Division of Marine Bioscience, Graduate School of Fi
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Miyashita Kazuo
Laboratory Of Biofunctional Material Chemistry Division Of Marine Bioscience Craduate School Of Fish
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Araseki Mina
Laboratory Of Biofunctional Material Chemistry Division Of Marine Bioscience Graduate School Of Fish
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Miyashita Kazuo
Laboratory Of Biofunctional Material Chemistry Division Of Marine Bioresources Graduate School Of Fi
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Miyashita Kazuo
Laboratory Of Biofunctional Material Chemistry Division Of Marine Bioscience Graduate School Of Fish
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Miyashita Kazuo
Laboratory of Bio-functional Material Chemistry, Division of Marine Bioscience, Faculty of Fisheries Sciences, Hokkaido University
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HOSOKAWA Masashi
Laboratory of Bio-functional Material Chemistry, Division of Marine Bioscience, Faculty of Fisheries Sciences, Hokkaido University
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