Effective Production of Microinjectable Blastocysts for Germ-Line Transmission of Embryonic Stem Cells
スポンサーリンク
概要
- 論文の詳細を見る
Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.
- 社団法人 日本実験動物学会の論文
著者
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HYUN Byung-Hwa
ICLAS Monitoring Subcenter Korea, Korea Research Institute of Bioscience and Biotechnology
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Han Ying-hao
Disease Model Research Center Biological Resource Center Kribb
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Han Ying-hao
韓国
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Lee Sang
School Of Life Sciences And Biotechnology Korea University
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LEE Dong-Seok
College of Natural Sciences, Kyungpook National University
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Hyun Byung-hwa
Iclas Monitoring Subcenter Korea Genetic Resources Center Korea Research Institute Of Bioscience And
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KIM Sun-Uk
Laboratory of Human Genomics
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HAN Ying-Hao
Laboratory of Human Genomics
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LEE Tae-Hoon
Department of Oral Biochemistry, College of Dentistry, Chonnam National University
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LEE Sang-Ho
Laboratory of Reproductive and Developmental Biology, School of Life Sciences and Biotechnology, Kor
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LEE Dong-Seok
Laboratory of Human Genomics
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YU Dae-Yeul
Laboratory of Human Genomics
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Lee Dong-seok
College Of Natural Sciences Kyungpook National University
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Kim Sun-uk
School Of Life Sciences And Biotechnology Korea University:disease Model Research Center Biological
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Lee S.
School Of Life Sciences And Biotechnology Korea University
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Lee D‐s
College Of Natural Sciences Kyungpook National University
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Kim Sun
Laboratory Of Human Genomics Korea Research Institute Of Bioscience And Biotechnology (kribb):labora
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Yu Dae-yeul
Disease Model Research Center Biological Resource Center Kribb
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Yu Dae
Laboratory Of Human Genomics Korea Research Institute Of Bioscience And Biotechnology (kribb)
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Lee Sang-ho
韓国
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Lee Tae-hoon
Department Of Oral Biochemistry College Of Dentistry Chonnam National University
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Lee Dong-seok
Laboratory Of Human Genetics Korea Research Institute Of Bioscience And Biotechnology (kribb)
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