Gene Expression Analysis in Human Gastric Cancer Cell Line Treated with Trichostatin A and S-Adenosyl-L-homocysteine Using cDNA Microarray(Biochemistry/Molecular Biology)

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概要

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• Trichostatin A (TSA) and S-adenosyl-L-homocysteine (AdoHcy) have been reported to affect histone modifications. To investigate the effects of two drugs that can reportedly affect chromatin remodeling, we analyzed the gene expression profiles of TSA and AdoHcy in a gastric cancer cell line using 14 K cDNA microarray. The significant analysis of microarray (SAM) identified 98 and 43 differentially expressed genes in TSA and AdoHcy treated sets, respectively, and selected genes were functionally classified. In the gastric cancer cell line, genes related to cell communication, cell growth/maintenance, and morphogenesis were highly expressed with TSA, and genes with cell growth/maintenance, metabolism, oxidoreductase activity were upregulated with AdoHcy. Genes downregulated with TSA included those controlling the cell cycle, cell growth/proliferation, DNA binding, and metabolism, whereas genes involved in calcium signaling, cell growth/proliferation, and metabolism were downregulated with AdoHcy. Furthermore, we identified the genes commonly expressed in both drug treatments. Compared to TSA, AdoHcy did not induce apoptosis in the SNU-16 gastric cancer cell line, and RT-PCR was performed for selective genes to confirm the microarray data. This gene expression profile analysis with TSA and AdoHcy should contribute to a greater understanding of the molecular mechanism of chromatin remodeling and cancer, and provide candidate genes for further studies involving the roles of histone modifications in gastric cancer.

• 公益社団法人日本薬学会の論文
• 2004-10-01

著者

• Byun Sang-soon

  Laboratory Of Human Genetics Korea Research Institute Of Bioscience And Biotechnology (kribb)

• Seo Sang-beom

  Cell & Developmental Biology And Dental Research Institute College Of Dentistry Seoul National U

• LEE Dong-Seok

  Laboratory of Human Genomics

• Jung Hai-young

  Laboratory Of Human Genetics Korea Research Institute Of Bioscience And Biotechnology (kribb)

• Yoo H‐s

  Laboratory Of Human Genetics Korea Research Institute Of Bioscience And Biotechnology (kribb)

• Yeom Young

  Laboratory Of Human Genetics Korea Research Institute Of Bioscience And Biotechnology (kribb)

• Lee Dong-seok

  Laboratory Of Human Genetics Korea Research Institute Of Bioscience And Biotechnology (kribb)

• LEE Heun-Sik

  Laboratory of Human Genetics, Korea Research Institute of Bioscience and Biotechnology (KRIBB)

• PARK Mie-Hee

  Laboratory of Human Genetics, Korea Research Institute of Bioscience and Biotechnology (KRIBB)

• YANG Suk-Jin

  Laboratory of Human Genetics, Korea Research Institute of Bioscience and Biotechnology (KRIBB)

• YOO Hyang-Sook

  Laboratory of Human Genetics, Korea Research Institute of Bioscience and Biotechnology (KRIBB)

• Park Mie-hee

  Laboratory Of Human Genetics Korea Research Institute Of Bioscience And Biotechnology (kribb)

• Yang Suk-jin

  Laboratory Of Human Genetics Korea Research Institute Of Bioscience And Biotechnology (kribb)

• Lee Heun-sik

  Laboratory Of Human Genetics Korea Research Institute Of Bioscience And Biotechnology (kribb)

関連論文

• Effective Production of Microinjectable Blastocysts for Germ-Line Transmission of Embryonic Stem Cells
• Gene Expression Analysis in Human Gastric Cancer Cell Line Treated with Trichostatin A and S-Adenosyl-L-homocysteine Using cDNA Microarray(Biochemistry/Molecular Biology)

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