Application of Sandwich Assay to Resonance Acoustic Microbalance with Naked-Embedded Quartz Biosensor for High-Selectivity Detection of C-Reactive Protein
スポンサーリンク
概要
- 論文の詳細を見る
The selectivity of the resonance acoustic microbalance with naked-embedded quartz (RAMNE-Q) biosensor addition to its original features (label-free, high-sensitivity, and replacement-free) was improved by applying a sandwich assay. We succeeded in the real-time monitoring of a frequency change due to a series of reactions: the nonspecific adsorption of streptococcal protein G (SPG), the oriented immobilization of an anti-C-reactive protein (anti-CRP) antibody, the specific binding of CRP as the antigen, and the specific binding of another anti-CRP antibody as the detection antibody. The detection of the low concentrations of CRP (100 pg/ml) and the anti-CRP antibody (1 μg/ml) was carried out repeatedly, and its repeatability was confirmed.
- 2013-07-25
著者
-
Ogi Hirotsugu
Graduate School Of Engineering Science Osaka University
-
Hirao Masahiko
Graduate School Of Engineering Sci. Osaka Univ.
-
Kato Fumihito
Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan
-
Tsurimoto Keisuke
Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan
関連論文
- Extraction of Interface Stiffness in Superlattices : Proposal of the Interface Elasticity Parameter
- Ultrasound-Induced Structural Anomaly of Supercooled Liquid in Some Bulk Metallic Glasses
- P1-1 Study on the Room-Temperature Aging of Cu Thin Films : Monitoring of Elasticity by Resonant-Ultrasound Spectroscopy(Poster session 1)
- OS6(5)-22(OS06W0137) Acoustic Spectroscopy for Measuring Anisotropic Elastic Constants of Thin Films
- Acoustic Study of Martensitic Phase Transformation in TiNbAl Shape Memory Alloy
- P2-43 Elastic and magnetic anisotropy in Co/Pt superlattices : Measurement by RUS/Laser method(Short presentation for poster)
- High-Frequency Electrodeless Quartz Crystal Microbalance Chip with a Bare Quartz Resonator Encapsulated in a Silicon Microchannel
- Elastic Constants of Single-Crystal Rare-Earth Bismuth Iron Garnet Films
- Fast Recovery of Elasticity of Cu Thin Films at Room Temperature Studied by Resonant-Ultrasound Spectroscopy
- Measurement of Elastic Constant and Refraction Index of Thin Films at Low Temperatures Using Picosecond Ultrasound
- P1-6 Excitation and Detection of Ultrasound Shear Wave by EMAR under High Pressure(Poster session 1)
- P1-8 Elastic Constants and Piezoelectric Coefficients of Langasite Single Crystal at Low Temperatures(Poster session 1)
- P2-30 Development of a high-sensitive electrodeless QCM immunosensor(Poster session 2)
- High Temperature Elastic Constants of α-Fe Single Crystal Studied by Electromagnetic Acoustic Resonance
- Label-Free Ultrathin Pt Film Biosensor Using Phonon Vibrations Excited by Ultrafast Light Pulses
- Fast Recovery of Elastic Stiffness in Ag Thin Film Studied by Resonant-Ultrasound Spectroscopy
- Detection of Glypican-3 Proteins for Hepatocellular Carcinoma Marker Using Wireless-Electrodeless Quartz-Crystal Microbalance
- Detection of shear horizontal guided waves propagating in aluminum plate with thinning region (Special issue: Ultrasonic electronics)
- Resonance Ultrasound Spectroscopy for Measuring Elastic Constants of Thin Films
- Pressure-Dependent Shear Modulus of Polycrystalline Al Studied by Electromagnetic Acoustic Resonance
- Development of 170 MHz Electrodeless Quartz-Crystal Microbalance Immunosensor with Nonspecifically Immobilized Receptor Proteins
- Resonant Ultrasound Microscopy with Isolated Langasite Oscillator for Quantitative Evaluation of Local Elastic Constant
- Noncontact Evaluation of Surface-Wave Nonlinearity for Creep Damage in Cr–Mo–V Steel
- Annealing Effect on Acoustic Property of Fe/Pt Superlattice Studied by Picosecond Ultrasound
- Monitoring of Longitudinal-Wave Velocity and Attenuation of SrTiO3 at Low Temperatures Using Picosecond Ultrasound Spectroscopy
- Picosecond Ultrasound Spectroscopy with a Stable Fiber Laser for Ultrahigh-Frequency-Oscillator Applications: from Nanomechanics to Biosensors
- Elastic Properties of Pd-Based Bulk Metallic Glasses Studied by Ultrasouond Spectroscopy
- Elastic-Constant Measurement in Oxide and Semiconductor Thin Films by Brillouin Oscillations Excited by Picosecond Ultrasound
- Aggregation Behavior of Amyloid $\beta_{1--42}$ Peptide Studied Using 55 MHz Wireless–Electrodeless Quartz Crystal Microbalance
- Elastic Constants and Magnetic Anisotropy of Co/Pt Superlattice: Resonance-Ultrasound-Spectroscopy Method
- Fabrication of Isolated FePd Nanoparticles by Sputtering and Heat Treatment
- Energy Trap for Axial-Shear-Wave Resonance in a Stepped Cylindrical Rod: Theory and Measurement
- Study of Elastic Anisotropy of Cu Thin Films by Resonant-Ultrasound Spectroscopy Coupled with Laser-Doppler Interferometry and Pump-Probe Photoacoustics
- Elastic Constants of Co/Pt Superlattice Studied by Acoustic Measurements and Ab initio Calculations
- Magnetic Phase Transition and Elastic Properties of $\alpha$-Mn at Low Temperature
- Increase of Efficiency of Magnetostriction SH-Wave Electromagnetic Acoustic Transducer by Angled Bias Field: Piezomagnetic Theory and Measurement
- High-Frequency Wireless and Electrodeless Quartz Crystal Microbalance Developed as Immunosensor
- Electromagnetic Acoustic Resonance to Assess Creep Damage in Cr–Mo–V Steel
- Effects of Dissolved and Ambient Gases on Sonochemical Degradation of Methylene Blue in High-Amplitude Resonant Mode
- Mechanisms of Ultrasonically Induced Fibrillation of Amyloid \beta_{1{\mbox{--}}40} Peptides
- Elastic Stiffness of Co Thin Films at High Temperatures Monitored by Picosecond Ultrasound
- Application of Sandwich Assay to Resonance Acoustic Microbalance with Naked-Embedded Quartz Biosensor for High-Selectivity Detection of C-Reactive Protein
- Mode Conversion and Total Reflection of Torsional Waves for Pipe Inspection
- Elastic Stiffness of Co Thin Films at High Temperatures Monitored by Picosecond Ultrasound (Special Issue : Ultrasonic Electronics) -- (Ultrasonic properties of materials, phonon physics, acousto-optics)