Atomic Force Microscopy Observation of Membrane Proteins Suspended over Carbon Nanotube Network
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概要
- 論文の詳細を見る
Atomic force microscopy (AFM) imaging of membrane proteins suspended over a nanostructure in liquid is a promising way to understand the structure and function of working proteins, although the membrane deformation that occurs during scanning makes it difficult to obtain a high resolution image. This study proposes an artificial cell system for the AFM observation of functional membrane proteins that consists of a sub-micron well on Si, a biological membrane, and a carbon nanotube (CNT) network. We successfully observed molecular-scale images of a purple membrane suspended over sub-micron well patterns. By using a CNT network to hold the suspended membrane, we suppressed the membrane deformation caused by the “imaging force”. The CNT network takes the place of a cytoskeleton in supporting the cell membrane suspended over the well thus improving the spatial resolution of AFM measurement.
- 2009-08-25
著者
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SUMITOMO Koji
NTT Basic Research Laboratories
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TORIMITSU Keiichi
NTT Basic Research Laboratories
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KOBAYASHI Yoshihiro
NTT Basic Research Laboratories
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Shinozaki Youichi
Ntt Basic Research Laboratories Ntt Corporation
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Nakashima Hiroshi
Ntt Basic Research Laboratories Ntt Corporation
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Takagi Daisuke
NTT Basic Research Laboratories, NTT Corporation, Atsugi, Kanagawa 243-0198, Japan
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Kobayashi Yoshihiro
NTT Basic Research Laboratories, NTT Corporation, Atsugi, Kanagawa 243-0198, Japan
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Shinozaki Youichi
NTT Basic Research Laboratories, NTT Corporation, Atsugi, Kanagawa 243-0198, Japan
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Nakashima Hiroshi
NTT Basic Research Laboratories, NTT Corporation, Atsugi, Kanagawa 243-0198, Japan
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Torimitsu Keiichi
NTT Basic Research Laboratories, NTT Corporation, Atsugi, Kanagawa 243-0198, Japan
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