Effect of Ca2+ on Vesicle Fusion on Solid Surface: An In vitro Model of Protein-Accelerated Vesicle Fusion

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概要

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• Lipid vesicle fusion is an important reaction in the cell. Calcium ions (Ca2+) participate in various important biological events including the fusion of vesicles with cell membranes in cells. We studied the effect of Ca2+ on the fusion of egg yolk phosphatidylcholine/brain phosphatidylserine (eggPC/brainPS) lipid vesicles on a mica substrate with fast scanning atomic force microscopy (AFM). When unattached and unfused lipid vesicles on mica were rinsed away, discrete patches of fused vesicles were observed under high Ca2+ concentrations. At 0 mM Ca2+, lipid vesicles were fused on mica and formed continuous supported lipid bilayers (SLBs) covering almost the entire mica surface. The effect of Ca2+ on SLB formation was offset by a Ca2+ chelating agent. When lipid vesicles were added during AFM observation, vesicles fused on mica and covered almost all areas even under high Ca2+ concentrations. These results indicate that force between AFM tip and vesicles overcomes the Ca2+-reduced fusion of lipid vesicles.

• 2008-07-25

著者

• SUMITOMO Koji

  NTT Basic Research Laboratories

• TORIMITSU Keiichi

  NTT Basic Research Laboratories

• FURUKAWA Kazuaki

  NTT Basic Research Laboratories

• Shinozaki Youichi

  Ntt Basic Research Laboratories Ntt Corporation

• Shinozaki Youichi

  NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Astugi, Kanagawa 243-0198, Japan

• Sumitomo Koji

  NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Astugi, Kanagawa 243-0198, Japan

• Siitonen Ari

  NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Astugi, Kanagawa 243-0198, Japan

• Torimitsu Keiichi

  NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Astugi, Kanagawa 243-0198, Japan

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