Confinement of Fluorescent Probes in Microwells on Si Substrates by Sealing with Lipid Bilayers
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概要
- 論文の詳細を見る
We investigated the optimum architecture for confining fluorescent probes in microwells on a Si substrate by covering it with a lipid bilayer. We modified the structure of the wells to prevent the lipid membrane from falling into them, and the overhang shape at the aperture improved the probability of confinement. The fluorescence intensity from the calcein confined in the wells remained unchanged for one hour or more, indicating that the probes remain stably in the wells without flowing out. An artificial cell sealed with the suspended membrane is a promising tool for the functional analysis of membrane proteins.
- Japan Society of Applied Physicsの論文
- 2010-10-25
著者
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SUMITOMO Koji
NTT Basic Research Laboratories
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TORIMITSU Keiichi
NTT Basic Research Laboratories
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Shinozaki Youichi
NTT Basic Research Labs.
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Tamba Yukihiro
Suzuka Nat. Coll. Tech.
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Torimitsu Keiichi
Ntt Basic Research Laboratories Ntt Corporation
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Tamba Yukihiro
Ntt Basic Research Laboratories Ntt Corporation
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Torimitsu Kenchi
Ntt Basic Research Laboratories Ntt Corporation
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Shinozaki Youichi
Ntt Basic Research Laboratories Ntt Corporation
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Sumitomo Koji
Ntt Basic Research Labs.
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Tamba Yukihiro
Suzuka Nat. Coll. of Tech.
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