Detection of DNA Hybridization Properties Using Thermodynamic Method

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The determination of DNA hybridization reaction can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and application area. So, the improvement of DNA hybridization detection method is very important for the determination of hybridization reaction. Several molecular biological techniques require accurate predictions of match versus mismatch hybridization thermodynamics, such as polymerase chain reaction (PCR), sequencing by hybridization, gene diagnostics and antisense DNA probes. In addition, recent developments of DNA chip array as means for biochemical assays and DNA sequencing requires accurate knowledge of hybridization thermodynamics and population ratios at match and mismatch target sites. In this study, we report the properties of the probe oligonucleotide and match, mismatch target oligonucleotide hybridization reaction using thermodynamic method. Thermodynamics of 5$'$-oligonucleotides with central and terminal mismatch sequences are obtained by measuring UV-absorbance as a function of temperature. The data show that the nearest-neighbor base-pair model is adequate for predicting thermodynamics of oligonucleotides with average deviations for enthalpy ($\Delta H^{0}$), entropy ($\Delta S^{0}$), free energy change at 37°C ($\Delta{G_{37}}^{0}$) and melting temperature ($T_{\text{m}}$), respectively.
Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
2006-01-15

Detection of DNA Hybridization Properties Using Thermodynamic Method

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