Development of a Functional Chromosome Nano-Dissection System Using Porous Anodic Alumina Pattern Chip and Cantilever
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概要
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The dissection of chromosomes followed by extraction of specific DNA fragments constitutes a topic of high interest, especially for cytogenetic studies in the post genome era. The dissected chromosomes can be used in various conventional applications, including establishing probes for fluorescence in situ hybridization (FISH), the generation of chromosome band-specific libraries and physical mapping for cytogenetic analysis. In the present study, we developed a functional chromosome nano-dissection system that can dissect various chromosomes in one-chip by using the pattern chip with porous anodic alumina. The porous anodic alumina, that makes use of self-organized nanoarray materials as a template for the pattern chip, has a highly oriented porous structure with uniform and nearly parallel pores that can be organized in an almost precise close-packed hexagonal structure. The most characteristic feature of this material has been emphasized with the extremely high aspect ratio of their channels that is difficult to achieve with conventional lithographic techniques. This chromosome nano-dissection system can dissect directly a specific chromosome into nano-pores on the porous anodic alumina (PAA) pattern chip without an atomic force microscope (AFM) dissection process. Thus, we expect that our PAA pattern chip is very suitable for arraying chromosome fragments without the recovery process and the possibility of direct immobilization of chromosome fragments into the nano-pores of this chip.
- 2007-04-30
著者
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KWON Young-Soo
Department of Electrical Engineering & NTRC, Dong-A University
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Tamiya Eiichi
School Of Chemical Materials Science Japan Advanced Institute Of Science And Technology
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Kim Do-kyun
School Of Materials Science Japan Advanced Institute Of Science & Technology
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Saito Masato
School Of Materials Science Japan Advanced Institute Of Science & Technology
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Kwon Young-Soo
Department of Electrical Engineering, Dong-A University, 840 Hadan-2dong, Saha-gu, Busan 604-714, Korea
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Kim Do-Kyun
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan
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