Polymer Size Effect on Shape and Position in DNA Trap by Electric and Hydrodynamic Force Fields
スポンサーリンク
概要
- 論文の詳細を見る
In recent years, a phenomenon has been discovered wherein charged particles are trapped near the constricted position of a microfluidic tapering channel when hydropressure and electric field are applied in opposite directions. By this phenomenon, the existence of various types of trapping patterns has already been demonstrated in a T4 phage DNA trapping experiment. In this study, we aim to explain the mechanism of this phenomenon. The relationship between DNA size and trapping pattern was examined using five different sizes of DNA samples. As a result, the trapping pattern and trapping condition extremely different with size when the most stable and clearest trapping image is observed. That is, the main trapping patterns change markedly in shapes and trapped positions, and large DNA traps occur under the conditions of low pressure and low voltage, whereas small size traps occur under the conditions of high pressure and high voltage. Another important result is that the ratio of the pressure and the voltage is independent of size when the main trap is observed. These findings suggest that this trap has size selectivity and molecular selectivity for extracting target molecules from a mixed solution.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2007-08-15
著者
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Tamiya Eiichi
School Of Chemical Materials Science Japan Advanced Institute Of Science And Technology
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Takamura Yuzuru
School Of Chemical Materials Science Japan Advanced Institute Of Science And Technology
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Nakamori Yoshiteru
School Of Knowledge Science Japan Advanced Institute Of Science And Technology
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Tamiya Eiichi
School of Material Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan
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Tomizawa Yuichi
School of Material Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan
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Ueno Kunimitsu
School of Knowledge Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan
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Nagasaka Wako
School of Material Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan
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Takamura Yuzuru
School of Material Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan
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