Propitious Immobilization of Gold Nanoparticles on Poly(dimethylsiloxane) Substrate for Local Surface Plasmon Resonance Based Biosensor
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概要
- 論文の詳細を見る
In this work, a simple method for fabricating gold nanoparticle (AuNP) layer on a poly(dimethylsiloxane) (PDMS) substrate based on electrostatic deposition of AuNP colloid onto a chemically-modified PDMS surface using 3-aminopropyltriethoxysilane (\gamma-APTES) was developed. AuNPs of 100 nm diameter were successfully dispersed and deposited onto the chemically-modified PDMS surface. The morphology and optical property of the AuNP layer were examined by atomic force microscopy (AFM) and UV--visible absorption spectroscopy, respectively. It was found that the prepared AuNP layer on PDMS could work as a localized surface plasma resonance (LSPR) sensor. The sensing characteristics were examined by changing the refractive index of solution surrounding the AuNP and antigen-antibody events on the AuNP surface. Changes in absorbance intensity and peak wavelength shift of the LSPR band were both clearly observed. The developed technique can hopefully expand the applications of PDMS for not only micro channel fabrication, but also sensing construction for easier and simpler preparation of microfluidic biosensors, which were then applied for immunoassays and other biochemical analyses.
- 2012-03-25
著者
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Trung Nguyen
School Of Materials Sci. Japan Advanced Inst. Of Sci. And Technol. (jaist) 1-1 Asahidai Nomi Ishikaw
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Tamiya Eiichi
Department Of Applied Physics Graduate School Of Engineering Osaka University
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Takamura Yuzuru
School Of Chemical Materials Science Japan Advanced Institute Of Science And Technology
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Yoshikawa Hiroyuki
Department Of Applied Physics And Handai Frontier Research Center Osaka University
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Trung Nguyen
School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), Nomi, Ishikawa 923-1292, Japan
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Viet Pham
Hanoi University of Science, 334 Nguyen Trai, Hanoi, Vietnam
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Ashahi Tsuyoshi
Graduate School of Science and Engineering, Ehime University, Matuyama 790-8577, Japan
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Tamiya Eiichi
Department of Applied Physics, Osaka University, Suita, Osaka 565-0871, Japan
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Yoshikawa Hiroyuki
Department of Applied Physics, Osaka University, Suita, Osaka 565-0871, Japan
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Takamura Yuzuru
School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), Nomi, Ishikawa 923-1292, Japan
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