Binding Evaluation of Targeted Microbubbles with Biotin–Avidin Interaction by Surface Plasmon Resonance Biosensor

概要

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Targeted microbubbles for ultrasound imaging were examined with an avidin-modified gold surface of a surface plasmon resonance (SPR) biosensor. Contrast media of ultrasound (US) imaging were fabricated with dispersions of biotinylated gases (microbubbles) in an aqueous medium and the binding interaction of a targeted microbubble contrast agent was characterized by the refractive index change of the adsorbed layers in the SPR biosensor. The shells of the microbubbles were consisted of a gas-filled layer of lipids and polypeptides. Avidin–biotin interaction as a typical protein–ligand model was investigated on biotinylated polypeptide lipid microbubbles that were functionalized with biotin by directly grafting them to poly(succinimide) (PSI). The targeted microbubbles were below 7 μm (95%) and their mean diameter was 2.5 μm. The affinity constant ($K_{\text{A}}$) of the biotinylated polypeptide lipid-microbubbles from the SPR data ($K_{\text{A}}=9.46$ g-1 L) was about 10 times greater than that of polypeptide lipid microbubbles ($K_{\text{A}}=1.00$ g-1 L). The SPR biosensor may help in the prediction of the in vivo behavior of targeted microbubbles (ultrasound contrast agent) in a flow system and in the design of successful target preparation.
Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
2006-01-15