Temperature-Sensitivity of Liposomal Lipid Bilayers Mixed with Poly(N-Isopropylacrylamide-co-acrylic Acid).
スポンサーリンク
概要
- 論文の詳細を見る
Temperature-sensitive drug release was examined using liposomes mixed with a copoly-mer of N-isopropylacrylamide (NIPAM) and acrylic acid [P(NIPAM-AA)] i. e., thermally responsive liposomes. P(NIPAM-AA) copolymers with transition temperatures of about 30, 33, 37, and 43°C were synthesized by copolymerizing NIPAM and acrylic acid. Thermally responsive liposomes were prepared by mixing hydrophobically modified PNIPAM, or P(NIPAM-AA) with various liposomes, composed of egg phosphatidylcholine (PC), dimy-ristoylphosphatidylcholine (DMPC)/dipalmitoylphosphatidylcholine (DPPC) mixture (5 : 5, w/w), DPPC, or distearoylphosphatidylcholine (DSPC). The release of a fluorescent marker, calcein, from liposomes was monitored by injecting the liposomal suspension at 17°C into phosphate-buffered saline (PBS, pH 7.4) preadjusted to a temperature ranging from 20 to 46°C. For liposomes of egg PC and DSPC, which do not undergo a phase transition during the temperature jump (17→20-46°C), the release temperature of the liposomes increased as the content of acrylic acid in the copolymers increased. The interaction between copolymer and lipid may induce the release of calcein at LCST of the copolymer. For DPPC liposomes, the release patterns were similar to those of egg PC and DSPC liposomes at 20-36°C, where the phase transition of the liposomal membrane did not occur, while at 36-46°C, where the phase transition of liposomal membrane occurred, the degree of release was almost the same. For DMPC/DPPC (5 : 5, w/w) liposomes, where the transition occurred below those of PNIPAMs, equally enhanced releases were observed as compared with PNIPAMs, even below the LCSTs of PNIPAMs. Thus, regardless of the occurrence of the transition of PNIPAMs, phase transition of DMPC/DPPC liposomes controlled the release of calcein.
- 社団法人 日本生化学会の論文
著者
-
KIM Jong-Duk
Department of Chem. Eng., Korean Advanced Institute of Science and Technology
-
Bae Soo
Department Of Chemical Engineering And Bioprocess Engineering Research Center Korea Advanced Institu
-
Kim Jin-chul
Department Of Chemical Engineering And Bioprocess Engineering Research Center Korea Advanced Institu
-
Kim Jin-Chul
Department of Chemical Engineering and Bioprocess Engineering Research Center, Korea Advanced Institute of Sczence and Technology
関連論文
- NON-ISOTHERMAL ADSORPTION OF NITROGEN-CARBON DIOXIDE MIXTURE IN A FIXED BED OF ZEOLITE-X
- Photorefractivity and Optical Transmittance of Hybrid Liquid Crystal Cell With Homeotropically Photoaligned Layer and Fullerene Initiation : Optical Properties of Condensed Matter
- Second Harmonic Generation and Photorefractive Effect in Dye-Doped Liquid Crystals : Optics and Quantum Electronics
- Palmitoyl Lysozyme-induced Stabilizatio of PE (Phosphatidylethanolamine0 Liposomes nad Their Interaction with Candida albicans
- Simultaneous quantification of rosiglitazone and its two major metabolites, N-desmethyl and p-hydroxy rosiglitazone in human plasma by liquid chromatography/tandem mass spectrometry : Application to a pharmacokinetic study
- Liquid chromatography/tandem mass spectrometry method for the simultaneous determination of vardenafil and its major metabolite, N-desethylvardenafil, in human plasma : Application to a pharmacokinetic study
- Temperature-Sensitivity of Loposomal Lipid Bilayers Mixed with Poly(N-Isopropylacrylamide-co-acrylic Acid)
- Grating Formation Time of Photorefractive Liquid Crystal Cell with Mixed Layer of PVK and $p$-PMEH-PPV
- Binding Evaluation of Targeted Microbubbles with Biotin–Avidin Interaction by Surface Plasmon Resonance Biosensor
- Self-Assembled Nanoparticles of Ribozymes with Poly(ethylene glycol)-$b$-Poly(l-lysine) Block Copolymers
- Kinetics of Reduction of Uranium (VI) to Uranium (IV) at Titanium Electrode in Nitric Acid and Hydrazine Media.
- Temperature-Sensitivity of Liposomal Lipid Bilayers Mixed with Poly(N-Isopropylacrylamide-co-acrylic Acid).
- Palmitoy Lysozyme-Induced Stabilization of PE (Phosphatidylethanolamine) Liposomes and Their Interaction with Candida albicans.