Paracellular Barrier and Tight Junction Protein Expression in the Immortalized Brain Endothelial Cell Lines bEND.3, bEND.5 and Mouse Brain Endothelial Cell 4
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概要
- 論文の詳細を見る
The blood–brain barrier (BBB) is formed by brain endothelial cells. Many immortalized brain endothelial cell lines have been established; these have been used as in vitro BBB models. The aim of the present study was to assess the paracellular barrier properties of the immortalized mouse brain endothelial cell lines bEND.3, bEND.5 cells, and mouse brain endothelial cell 4 (MBEC4), and those of the primary mouse brain endothelial cells pMBECs. bEND.3 cells showed low permeability to sodium fluorescein and obvious staining of tight junction proteins (claudin-5, occludin and ZO-1) similar to pMBECs; these barrier properties of MBEC4 and bEND.5 cells were low. In addition, bEND.3 cells expressed the highest level of claudin-5 among all cells. These results suggest that bEND.3 cells are a convenient and useful model for evaluating BBB function, especially the paracellular barrier.
- 公益社団法人 日本薬学会の論文
著者
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Watanabe Takuya
Department Of Biochemistry Showa University School Of Medicine
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Nakashima Akio
Department Of Chemistry Faculty Of Science Shizuoka University
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Takata Fuyuko
Department Of Pharmaceutical Care And Health Sciences Faculty Of Pharmaceutical Sciences Fukuoka Uni
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Kataoka Yasufumi
Department Of Hospital Pharmacy Faculty Of Medicine Kyushu University
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Futagami Koujiro
Department Of Hospital Pharmacy Faculty Of Medicine Kyushu University
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Yamauchi Atsushi
Department Of Biology Faculty Of Science Kyushu University
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Dohgu Shinya
Department Of Pharmaceutical Care And Health Sciences Faculty Of Pharmaceutical Sciences Fukuoka Uni
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Nishioku Tsuyoshi
Department Of Pharmaceutical Care And Health Sciences Faculty Of Pharmaceutical Sciences Fukuoka University
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Kataoka Yasufumi
Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University
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Nishioku Tsuyoshi
Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University
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Takata Fuyuko
Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University
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Futagami Koujiro
Department of Pharmaceutical and Health Care Management, Faculty of Pharmaceutical Sciences, Fukuoka University
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