Interaction of PICK1 with C-Terminus of Growth Hormone–Releasing Hormone Receptor (GHRHR) Modulates Trafficking and Signal Transduction of Human GHRHR
スポンサーリンク
概要
- 論文の詳細を見る
Release of growth hormone (GH) from the somatotroph is regulated by binding GH-releasing hormone (GHRH) to its cognate receptor (GHRHR), one of the members of the G protein–coupled receptor (GPCR) superfamily. Proteins bound to the carboxy (C)-terminus of GPCR have been reported to regulate intracellular trafficking and function of the receptor; however, no functionally significant protein associated with GHRHR has been reported. We have identified a protein interacting with C-kinase 1 (PICK1) as a binding partner of GHRHR. In vitro binding assay revealed the PDZ-domain of PICK1 and the last four amino acid residues of GHRHR were prerequisite for the interaction. Further, in vivo association of these proteins was confirmed. Immunostaining data of a stable cell line expressing GHRHR with or without PICK1 suggested the C-terminus of GHRHR promoted cell surface expression of GHRHR and PICK1 affected the kinetics of the cell surface expression of GHRHR. Furthermore, cAMP production assay showed the C-terminus of GHRHR is involved in the regulation of receptor activation, and the interaction of GHRHR with PICK1 may influence intensities of the signal response after ligand stimulation. Thus, the interaction of the C-terminus of GHRHR with PICK1 has a profound role in regulating the trafficking and the signaling of GHRHR.
著者
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Fukunaga Kohji
Department Of Pharmacology Graduate School Of Pharmaceutical Sciences Tohoku University
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Katsushima Yuriko
Department Of Pediatrics Tohoku University School Of Medicine
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Yanagisawa Teruyuki
Department Of Molecular Pharmacology Tohoku University Graduate School Of Medicine
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Sato Takeya
Department Of Anatomy Akita University School Of Medicine
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Ogawa Eishin
Department Of Pediatrics Tohoku University Hospital
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Sukegawa Jun
Department Of Cancer Prevention The Institute Of Medical Science The University Of Tokyo
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Suzuki Yuki
Department Of Applied Chemistry Graduate School Of Engineering Osaka Prefecture University
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Suzuki Yuki
Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Japan
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Yamada Chika
Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Japan
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Ito Moe
Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Japan
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Sukegawa Izumi
Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Japan
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Sukegawa Jun
Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Japan
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Fukunaga Kohji
Department of Pharmacology, Tohoku University Graduate School of Pharmaceutical Sciences, Japan
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Katsushima Yuriko
Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Japan
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Yanagisawa Teruyuki
Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Japan
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