DEVELOPMENT OF CELL-CELL JUNCTIONS IN FIFFERENT TYPES OF EXTRACELLULAR MATRICES : AN ANALYSIS IN CULTURED HEPATIC STELLATE CELLS IN THE PRESENCE OF A LONG-ACTING VITAMIN C DERIVATIVE

概要

論文の詳細を見る
Co-cultures of human differentiated hepatoma cells (Hep G2 cells) and human stellate cells (LI90), in the presence of a long-acting vitamin C derivative, L-ascorbic acid 2-phosphate (Asc 2-P), and incubated on type I collagen gel by using a conventional" smgle gel culture technique" and a "double gel culture technique", formed three main components of the junctional complex, i.e., tight junctions, adherens junctions, and desmosomes, between the Hep G2 cells. These three types of intercellular junctional components participate in forming of a bile canalicular system in the liver lobules by the formation of the junctional complexes between hepatic parenchymal cells in vivo. Gap junctions were formed between the Hep G2 cells in the double gel cultures. In this culture medium Hep G2 cells reproduced cell-cell junctional devices as seen in vivo. To investigate intercellular junctions between hepatic stellate cells, we cultured LI90 cells alone in the presence of Asc 2-P in non-coated polystyrene culture dishes, on type I collagen gel, or on Matrigel. Intercellular junctions were detected between these cultured LI90 cells, and these junctions were identified as adherens junctions. Stellate cells coexist with many kinds of extracellular matrix components within the normal perisinusoidal space. The junctions developed in the LI90 cells irrespective of the type of culture substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro. Thus, the hepatic stellate cells may participate in the structural organization of the cells in liver lobules through the formation of intercellular junctions between themselves. This report is the first description of the presence of cell-cell junctions between hepatic stellate cells in mammals at the fine structural level.
日本結合組織学会の論文

DEVELOPMENT OF CELL-CELL JUNCTIONS IN FIFFERENT TYPES OF EXTRACELLULAR MATRICES : AN ANALYSIS IN CULTURED HEPATIC STELLATE CELLS IN THE PRESENCE OF A LONG-ACTING VITAMIN C DERIVATIVE

スポンサーリンク

概要

著者

関連論文

スポンサーリンク