Autophosphorylation of Ca2+/calmodulin-dependent protein kinase. II. Effects on interaction between enzyme and substrate.
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概要
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Characteristics of the autophosphorylation of Ca<SUP>2+</SUP>/calmodulin-dependent protein kinase II (CaM kinase II) from the cytosol and in the postsynaptic densities (PSD) of rat brain were investigated. Several proteins were surveyed for their abilities to serve as a substrare for non-autophosphorylated and autophosphorylated CaM kinase Its from the cytosol and PSD. The tested substrates were separated into two groups. Autophosphorylation of the kinase slightly decreased or did not change its activities towards substrates of the first group: myosin light chain of chicken gizzard, synapsin I, tau factor and microtuble-associated protein 2. In contrast, autophosphorylation of the enzyme increased its activities towards substrates of the second group: syntide-2, histone H1, calcineurin and myelin basic protein. The Ca<SUP>2+</SUP>/calmodulin-independent kinase activity increased by autophosphorylation with any of substrates tested. Similar results were obtained with the cytosolic and PSD CaM kinase II. Trifluoperazine and mastoparan, calmodulin binding antagonists, inhibited the activity of the non-autophosphorylated CaM kinase II, but had no effect or only a slight inhibitory effect on the activity of the autophosphorylated CaM kinase II, indicating that the autophosphorylated kinase has no requirement for calmodulin for Ca<SUP>2+</SUP>dependent activity and/or a higher affinity for calmodulin The results suggest that the autophosphorylation of CaM kinase II is a subtle mechanism for regulating the interaction between the enzyme and substrate.
- 公益社団法人 日本薬理学会の論文
著者
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Fukunaga Kohji
Department Of Pharmacology Graduate School Of Pharmaceutical Sciences Tohoku University
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Miyamoto Eishichi
Department Of Electrical Engineering And Bioscience Graduate School Of Science And Engineering Wased
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Miyakawa Taihei
Department Of Neuropsychiatry Kumamoto University Medical School
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Yamamoto Hideyuki
Department Of Biochemistry Graduate School Of Medicine University Of The Ryukyus
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Yasugawa Setsuko
Department of Neuropsychiatry, Kumamoto University Medical School
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