Elimination of Interference of Maltose for the Determination of 1, 5-Anhydroglucitol with Lana AG<SUP>®</SUP> Kit (I)

概要

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We discovered and reported that the intravenous infusion of maltose interfered with the Lana AG<SUP>®</SUP> kit used for the determination of 1, 5-anhydroglucitol in plasma or serum. The principle of the kit consists of eliminating the interfering substances with a mini-column, an oxidation of the recovered 1, 5-anhydroglucitol by pyranose oxidase and a detection of the hydrogen peroxide with a color development reaction. In normal samples, glucose is a major interfering sugar in the detection system, but it is completely trapped in the mini-column. While a slight quantity of the infused maltose leaked out of the mini-column, the leaked maltose was hydrolyzed into glucose with a slight amount of maltase being present in pyranose oxidase and the formed glucose was oxidised with pyranose oxidase and changed color. To activate the affinity of the mini-column for maltose, we tried some conditioning methods for the mini-column. Washing the mini-column with a 10% ethylene glycol solution was the most effective method for the activation. The infused maltose was complete ly trapped in the conditioned mini-column and did not interfere with the determination of 1, 5-anhydroglucitol.
Japan Society of Clinical Chemistryの論文